Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/341610
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampLucke, Letícia Drudi-
dc.contributor.authorunicampBortolazzo, Fernanda Oriani-
dc.contributor.authorunicampBombeiro, André Luis-
dc.contributor.authorunicampCartarozzi, Luciana Politti-
dc.contributor.authorunicampVicente, Cristina Pontes-
dc.contributor.authorunicampOliveira, Alexandre Leite Rodrigues de-
dc.contributor.authorunicampPimentel, Edson Rosa-
dc.contributor.authorunicampAro, Andrea Aparecida de-
dc.typeArtigopt_BR
dc.titleLow-level laser and adipose-derived stem cells altered remodelling genes expression and improved collagen reorganization during tendon repairpt_BR
dc.contributor.authorLucke, Leticia D.-
dc.contributor.authorBortolazzo, Fernanda O.-
dc.contributor.authorTheodoro, Viviane-
dc.contributor.authorFujii, Lucas-
dc.contributor.authorBombeiro, Andre L.-
dc.contributor.authorFelonato, Maira-
dc.contributor.authorDalia, Rodrigo A.-
dc.contributor.authorCarneiro, Giane D.-
dc.contributor.authorCartarozzi, Luciana P.-
dc.contributor.authorVicente, Cristina Pontes-
dc.contributor.authorOliveira, Alexandre L. R.-
dc.contributor.authorMendonca, Fernanda A. S.-
dc.contributor.authorEsquisatto, Marcelo A. M.-
dc.contributor.authorPimentel, Edson R.-
dc.contributor.authorde Aro, Andrea A.-
dc.subjectDecorinapt_BR
dc.subject.otherlanguageDecorinpt_BR
dc.description.abstractThe cellular therapy using adipose-derived mesenchymal stem cells (ASCs) aims to improve tendon healing, considering that repaired tendons often result in a less resistant tissue. Our objective was to evaluate the effects of the ASCs combination with a low-level laser (LLL), an effective photobiostimulation for the healing processes. Rats calcaneal tendons were divided into five groups: normal (NT), transected (T), transected and ASCs (SC) or LLL (L), or with ASCs and LLL (SCL). All treated groups presented higher expression of Dcn and greater organization of collagen fibres. In comparison with T, LLL also up-regulated Gdf5 gene expression, ASCs up-regulated the expression of Tnmd, and the association of LLL and ASCs down-regulated the expression of Scx. No differences were observed for the expression of Il1b, Timp2, Tgfb1, Lox, Mmp2, Mmp8 and Mmp9, neither in the quantification of hydroxyproline, TNF-alpha, PCNA and in the protein level of Tnmd. A higher amount of IL-10 was detected in SC, L and SCL compared to T, and higher amount of collagen I and III was observed in SC compared to SCL. Transplanted ASCs migrated to the transected region, and all treatments altered the remodelling genes expression. The LLL was the most effective in the collagen reorganization, followed by its combination with ASCs. Further investigations are needed to elucidate the molecular mechanisms involved in the LLL and ASCs combination during initial phases of tendon repairpt_BR
dc.relation.ispartofCell proliferationpt_BR
dc.relation.ispartofabbreviationCell prolif.pt_BR
dc.publisher.cityChichesterpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherWileypt_BR
dc.date.issued2019-
dc.date.monthofcirculationMaypt_BR
dc.language.isoengpt_BR
dc.description.volume52pt_BR
dc.description.issuenumber3pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0960-7722pt_BR
dc.identifier.eissn1365-2184pt_BR
dc.identifier.doi10.1111/cpr.12580pt_BR
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/cpr.12580pt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumbernão tempt_BR
dc.description.sponsordocumentnumbernão tempt_BR
dc.date.available2020-05-18T16:05:21Z-
dc.date.accessioned2020-05-18T16:05:21Z-
dc.description.provenanceSubmitted by Mariana Aparecida Azevedo (mary1@unicamp.br) on 2020-05-18T16:05:21Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:16:21Z : No. of bitstreams: 1 000469271700022.pdf: 1565786 bytes, checksum: 1269ccf19f3d255b3293e4d6d59d7f92 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-18T16:05:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/341610-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordCell therapypt_BR
dc.subject.keywordGDF-5pt_BR
dc.subject.keywordHealingpt_BR
dc.subject.keywordPhotobiostimulationpt_BR
dc.subject.keywordTenomodulinpt_BR
dc.identifier.source000469271700022pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0003-2303-8127pt_BR
dc.creator.orcid0000-0003-1942-800Xpt_BR
dc.creator.orcid0000-0001-9896-4630pt_BR
dc.creator.orcid0000-0003-4224-4575pt_BR
dc.creator.orcid0000-0002-1049-8880pt_BR
dc.creator.orcid0000-0003-2895-0000pt_BR
dc.type.formArtigo originalpt_BR
dc.identifier.articleide12580pt_BR
dc.description.sponsorNoteThe authors thank CAPES and Hermínio Ometto University Center‐ UNIARARAS, for the financial support, Guilherme F. Caetano for his assistance with the ASCs differentiation protocol, and Maria Esméria C. do Amaral and Daniella N. R. Neodini for their technical assistance during Western blotting analysept_BR
Appears in Collections:IB - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000469271700022.pdf1.53 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.