Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/341588
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampDuran Caballero, Nelson Eduardo-
dc.typeArtigopt_BR
dc.titleBiosynthesis of CdS quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragipt_BR
dc.contributor.authorGallardo-Benavente, Carla-
dc.contributor.authorCarrion, Ornella-
dc.contributor.authorTodd, Jonathan D.-
dc.contributor.authorPieretti, Joana C.-
dc.contributor.authorSeabra, Amedea B.-
dc.contributor.authorDuran, Nelson-
dc.contributor.authorRubilar, Olga-
dc.contributor.authorPerez-Donoso, Jose M.-
dc.contributor.authorQuiroz, Andres-
dc.subjectPontos quânticospt_BR
dc.subjectBiossíntesept_BR
dc.subject.otherlanguageQuantum dotspt_BR
dc.subject.otherlanguageBiosynthesispt_BR
dc.description.abstractPreviously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H2S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1(T): megL(-) (unable to produce MeSH from Met) and mddA(-) (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL(-) strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA(-) strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur source. This work represents the first report that directly associates the production of MeSH with the bacterial synthesis of QDs, thus revealing the importance of different VSCs in the biological generation of metal sulfide nanostructurespt_BR
dc.relation.ispartofFrontiers in microbiologypt_BR
dc.relation.ispartofabbreviationFront. microbiol.pt_BR
dc.publisher.cityLausannept_BR
dc.publisher.countrySuiçapt_BR
dc.publisherFrontiers Research Foundationpt_BR
dc.date.issued2019-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume10pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1664-302Xpt_BR
dc.identifier.doi10.3389/fmicb.2019.01866pt_BR
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.01866/fullpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber404815/2018-9pt_BR
dc.description.sponsordocumentnumber2018/08194-2; 2018/02832-7pt_BR
dc.date.available2020-05-18T11:38:36Z-
dc.date.accessioned2020-05-18T11:38:36Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2020-05-18T11:38:36Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2020-05-18T11:38:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/341588-
dc.contributor.departmentDepartamento de Química Orgânicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.subject.keywordAntarctic bacteriapt_BR
dc.subject.keywordVolatile sulfur compoundspt_BR
dc.identifier.source000480537700004pt_BR
dc.creator.orcid0000-0001-8372-5143pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.identifier.articleid1866pt_BR
dc.description.sponsorNoteCONICYTComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) [21151066]; FondecytComision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)CONICYT FONDECYT [1151255, 1181697]; INACH [DT_05_16, RT-25_16]; FAPESPFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2018/08194-2, 2018/02832-7]; CNPqNational Council for Scientific and Technological Development (CNPq) [404815/2018-9]; Natural Environment Research CouncilNERC Natural Environment Research Council [NE/M004449]pt_BR
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