Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/341428
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCerqueira, Michael Antunes-
dc.contributor.authorunicampFerrari, Karen Linares-
dc.contributor.authorunicampMattos, Amilcar Castro de-
dc.contributor.authorunicampReis, Leonardo Oliveira-
dc.typeArtigopt_BR
dc.titleT cells CD4+/CD8+ local immune modulation by prostate cancer hemi-cryoablationpt_BR
dc.contributor.authorCerqueira, M.A.-
dc.contributor.authorFerrari, K.L.-
dc.contributor.authorde Mattos, A.C.-
dc.contributor.authorMonti, C.R.-
dc.contributor.authorReis, L.O.-
dc.subjectNeoplasias da próstatapt_BR
dc.subject.otherlanguageProstatic neoplasmspt_BR
dc.description.abstractPurpose: Tumors escape from the immune system by decreasing CD8+ and increasing CD4+ T cells’ activity, druggable targets. Thermal ablation might activate tumor-specific T cells by raising the presentation of tumor-specific antigens and hindering tumor negative immune regulation. Our aim was to assess T cell infiltrate pre- and post-cryoablation in a prospective observational study. Methods: A total of 240 sextant prostate biopsies cores (12 cores/patient) were collected from 10 unilateral prostate cancer patients (T1c, PSA density < 0.15 ng/dL, Gleason grade group 1, ≤ 2 cancer biopsy cores, and < 50% cancer core involvement) at diagnosis and 12 months after hemi-cryoablation. Cancer-positive (Diag+) and cancer-negative (Diag−) lobes at diagnosis and the same areas 12 months after hemi-cryoablation (Cryo+ and Cryo−, respectively) were explored by immunohistochemistry for infiltrating CD4+ and CD8+ T cells (in 45 random fields per prostate lobe, 400× magnification). The quantitative analysis of cells/mm2 and CD4+/CD8+ ratio were performed and compared among Diag+, Diag−, Cryo+, and Cryo− using ImageJ software. Results: There was a significant increase in tumor-infiltrating CD8+ T cells/mm2 in the Cryo+ tissue (mean, SD 0.31, 0.30) compared to Diag+ (0.18, 0.15), p = 0.015; confirmed in prostate acini (hot spots), p = 0.029, in which infiltrating CD4+/CD8+ T cells’ ratio decreased after hemi-cryoablation, p = 0.006. Infiltrating CD4+ T cells/mm2 presented a trend to decrease in Cryo+ (0.26, 0.27) compared to Diag+ (0.38, 0.32). Conclusions: This is the first study to show local immune modulation after prostate cancer cryoablation, characterized by decreasing CD4+/CD8+ T cells’ ratio, potential for clinical impact by unleashing the T-cell response to cancer. Future studies are necessary to explore different energies and longer follow-up clinical endpoints.pt_BR
dc.relation.ispartofWorld journal of urologypt_BR
dc.relation.ispartofabbreviationWorld j. urol.pt_BR
dc.publisher.cityHeidelbergpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherSpringerpt_BR
dc.date.issued2020-
dc.date.monthofcirculationMar.pt_BR
dc.language.isoengpt_BR
dc.description.volume38pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage673pt_BR
dc.description.lastpage680pt_BR
dc.rightsFechadopt_BR
dc.sourceScopuspt_BR
dc.identifier.issn0724-4983pt_BR
dc.identifier.eissn1433-8726pt_BR
dc.identifier.doi10.1007/s00345-019-02861-0pt_BR
dc.identifier.urlhttps://link.springer.com/article/10.1007/s00345-019-02861-0pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsordocumentnumber302622/2015-2pt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.date.available2020-05-14T17:30:27Z-
dc.date.accessioned2020-05-14T17:30:27Z-
dc.description.provenanceSubmitted by Bruna Maria Campos da Cunha (bcampos@unicamp.br) on 2020-05-14T17:30:27Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:18:07Z : No. of bitstreams: 1 2-s2.0-85069480725.pdf: 1395507 bytes, checksum: e33202ce15ce6003e0030772614e1ab8 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-14T17:30:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2020en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/341428-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.identifier.source2-s2.0-85069480725pt_BR
dc.creator.orcidorcid.org/0000-0002-9268-8484pt_BR
dc.creator.orcidorcid.org/0000-0002-7745-2123pt_BR
dc.creator.orcidorcid.org/0000-0002-8547-3530pt_BR
dc.creator.orcidorcid.org/0000-0003-2092-414Xpt_BR
dc.type.formArtigo originalpt_BR
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