Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/341119
Type: Artigo
Title: Gold nanoparticles for X-ray microtomography of neurons
Author: Depannemaecker, Damien
Santos, Luiz E. Canton
Almeida, Antonio-Carlos Guimarães de
Ferreira, Gustavo B. S.
Baraldi, Giovanni L.
Miqueles, Eduardo X.
Carvalho, Murilo de
Costa, Gabriel Schubert Ruiz
Marques, Marcia J. Guimarães
Scorza, Carla A.
Rinkel, Jean
Abstract: Commonly used methods to visualize the biological structure of brain tissues at subcellular resolution are confocal microscopy and two-photon microscopy. Both require slicing the sample into sections of a few tens of micrometers. The recent developments in X-ray microtomography enable three-dimensional imaging at sub-micrometer and isotropic resolution with larger biological samples. In this work, we developed and compared original microtomography methods and staining protocols to improve the contrast for in vitro mouse neuron imaging. Using Golgi's method to stain neurons randomly, we imaged the whole set of mouse brain structures. For specific and nonrandom neuron labeling, we conjugated 20 nm gold nanoparticles to antibodies used in the immunohistochemistry (IHC) method, using anti-NeuN to label specifically neuronal nuclei. We applied an original subtraction dual-energy method for microtomography in the vicinity of the Au L-III absorption edge and compared image reconstructions to confocal microscopy images acquired on the same samples. The results show the possibility to characterize the 3D entire brain structure of mice. They demonstrated a high contrast and neuron detection improvement by applying the dual-energy method coupled to IHC staining.
Subject: Cérebro - Imagem
Tomografia
Microtomografia por raio-X
Síncrotron
Imuno-histoquímica
Nanopartículas de ouro
Brain - Imaging
Tomography
X-ray microtomography
Synchrotron
Immunohistochemistry
Gold nanoparticles
Country: Estados Unidos
Editor: American Chemical Society
Rights: fechado
Identifier DOI: 10.1021/acschemneuro.9b00290
Address: https://pubs.acs.org/doi/10.1021/acschemneuro.9b00290
Date Issue: 2019
Appears in Collections:IFGW - Artigos e Outros Documentos

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