Please use this identifier to cite or link to this item:
Type: Artigo
Title: Castration-induced prostate epithelial cell apoptosis results from targeted oxidative stress attack of M1(142)-macrophages
Author: Barbosa, G. O.
Silva, J. A. F.
Siqueira-Berti, A.
Nishan, U.
Rosa-Ribeiro, R.
Oliveira, S. B. P.
Baratti, M. O.
Ferrucci, D.
Santana, J. C. O.
Damas-Souza, D. M.
Bruni-Cardoso, A.
Augusto, T. M.
Correa-da-Silva, F.
Moraes-Vieira, P. M.
Stach-Machado, D. R.
Felisbino, S. L.
Menezes, G. B.
Cesarz, C. L.
Carvalho, H. F.
Abstract: Prostate development and function are regulated by androgens. Epithelial cell apoptosis in response to androgen deprivation is caspase-9-dependent and peaks at Day 3 after castration. However, isolated epithelial cells survive in the absence of androgens. Znf142 showed an on-off expression pattern in intraepithelial CD68-positive macrophages, with the on-phase at Day 3 after castration. Rats treated with gadolinium chloride to deplete macrophages showed a significant drop in apoptosis, suggesting a causal relationship between macrophages and epithelial cell apoptosis. Intraepithelial M1-polarization was also limited to Day 3, and the inducible nitric oxide synthase (iNOS) knockout mice showed significantly less apoptosis than wild-type controls. The epithelial cells showed focal DNA double-strand breaks (DSB), 8-oxoguanine, and protein tyrosine-nitrosylation, fingerprints of exposure to peroxinitrite. Cultured epithelial cells induced M1-polarization and showed focal DSB and underwent apoptosis. The same phenomena were reproduced in LNCaP cells cocultured with Raw 264.7 macrophages. In conclusion, the M1 (142)-macrophage (named after Znf142) attack causes activation of the intrinsic apoptosis pathway in epithelial cells after castration.
Subject: Apoptose
Células epiteliais
Epithelial cells
Country: Estados Unidos
Editor: John Wiley & Sons
Rights: fechado
Identifier DOI: 10.1002/jcp.28544
Date Issue: 2019
Appears in Collections:IFGW - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000474688200198.pdf2.59 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.