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Type: Artigo
Title: Determination of short chain fatty acids in mice feces by capillary electrophoresis
Author: Marques, Leticia A.
Cazarin, Cinthia B. B.
Bicas, Juliano
Marostica Junior, Mario R.
Carrilho, Emanuel
Bogusz Junior, Stanislau
Abstract: Short-chain fatty acids (SCFA) are compounds produced during fermentation of gut microbiota. Acetic, propionic and butyric acids are the most important SCFA produced from non-digestible foods. We developed and validated a rapid and low-cost capillary electrophoretic (CE) method for determination of acetic, propionic and butyric acids in mice feces (100 mg of sample). Electrophoretic separation was performed for SCFA determination in feces samples during 10 min. The method showed good linearity for all analytes (determination coefficient, r(2) > 0.98); recovery from 74.1 to 109.8%, while the intra- and interday precision essays were suitable (relative standard deviation (RSD) < 10%); and limits of detection and quantification of 0.13-0.43 mM for acetic acid, 0.09-0.29 mM for propionic acid and 0.03-0.09 mM for butyric acid. Real samples of mice dextran sulfate sodium-induced colitis feces treated with jabuticaba (Plinia cauliflora) aqueous extract were performed successfully
Subject: Ácidos graxos de cadeia curta
Country: Brasil
Editor: Sociedade Brasileira de Química
Rights: Aberto
Identifier DOI: 10.21577/0103-5053.20190031
Date Issue: 2019
Appears in Collections:IQ - Artigos e Outros Documentos

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