Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/340789
Type: Artigo
Title: Facial selectivity between carbohydrates and aromatic amino acids explained by a combination of NCI, NBO and EDA techniques with NMR spectroscopy
Author: Rozada, Thiago C.
de Melo, Ulisses Z.
Pontes, Rodrigo M.
Rittner, Roberto
Basso, Ernani A.
Abstract: The influence of electrostatic and dispersion components of intermolecular interactions on the recognition of carbohydrates by aromatic protein residues is important for many biological processes. Interactions between glucose and galactose and aromatic moieties of tryptophan, phenylalanine and histidine were investigated through H-1 nuclear magnetic resonance (NMR) chemical shift perturbation and fully explained by molecular modelling at the density functional theory (DFT) level. According to NMR experiments, aromatic amino acids interact preferably with one face of the carbohydrate and the calculations showed how intermolecular interactions were determinant in explaining the selectivity. Non-covalent interaction surfaces revealed that a CH bond oriented toward the center of the aromatic ring maximized the attractive interaction while minimizing the steric repulsion. Energy decomposition analyses showed that the dispersion component was stronger than the electrostatic component and contributes more when hydrogen bonds are not present in the studied complexes. However, it was the electrostatic component that correlated with the facial preference, especially for the complexes with tryptophan
Subject: Carboidratos
Aminoácidos
Cálculos teóricos
Country: Brasil
Editor: Sociedade Brasileira de Química
Rights: Aberto
Identifier DOI: 10.21577/0103-5053.20180241
Address: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000500948
Date Issue: 2019
Appears in Collections:IQ - Artigos e Outros Documentos

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