Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/340460
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCamargo, Antônio Pedro-
dc.contributor.authorunicampNakahara, Thiago Seike-
dc.contributor.authorunicampMartins Netto, Paulo Henrique-
dc.contributor.authorunicampCarazzolle, Marcelo Falsarella-
dc.contributor.authorunicampPapes, Fabio-
dc.typeArtigopt_BR
dc.titleUncovering the mouse olfactory long non-coding transcriptome with a novel machine-learning modelpt_BR
dc.contributor.authorCamargo, Antonio P.-
dc.contributor.authorNakahara, Thiago S.-
dc.contributor.authorFirmino, Luiz E. R.-
dc.contributor.authorNetto, Paulo H. M.-
dc.contributor.authordo Nascimento, Joao B. P.-
dc.contributor.authorDonnard, Elisa R.-
dc.contributor.authorGalante, Pedro A. F.-
dc.contributor.authorCarazzolle, Marcelo F.-
dc.contributor.authorMalnic, Bettina-
dc.contributor.authorPapes, Fabio-
dc.subjectOlfatopt_BR
dc.subject.otherlanguageSmellpt_BR
dc.description.abstractVery little is known about long non-coding RNAs (lncRNAs) in the mammalian olfactory sensory epithelia. Deciphering the non-coding transcriptome in olfaction is relevant because these RNAs have been shown to play a role in chromatin modification and nuclear architecture reorganization, processes that accompany olfactory differentiation and olfactory receptor gene choice, one of the most poorly understood gene regulatory processes in mammals. In this study, we used a combination of in silico and ex vivo approaches to uncover a comprehensive catalogue of olfactory lncRNAs and to investigate their expression in the mouse olfactory organs. Initially, we used a novel machine-learning lncRNA classifier to discover hundreds of annotated and unannotated lncRNAs, some of which were predicted to be preferentially expressed in the main olfactory epithelium and the vomeronasal organ, the most important olfactory structures in the mouse. Moreover, we used whole-tissue and single-cell RNA sequencing data to discover lncRNAs expressed in mature sensory neurons of the main epithelium. Candidate lncRNAs were further validated by in situ hybridization and RT-PCR, leading to the identification of lncRNAs found throughout the olfactory epithelia, as well as others exquisitely expressed in subsets of mature olfactory neurons or progenitor cellspt_BR
dc.relation.ispartofDNA Researchpt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherOxford University Presspt_BR
dc.date.issued2019-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume26pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage365pt_BR
dc.description.lastpage378pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1340-2838pt_BR
dc.identifier.eissn1756-1663pt_BR
dc.identifier.doi10.1093/dnares/dsz015pt_BR
dc.identifier.urlhttps://academic.oup.com/dnaresearch/article/26/4/365/5535672pt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2015/50371-0; 2016/24471-0; 2013/08293-7; 2016/05379-6; 2018/11860-4; 2011/22611-6pt_BR
dc.date.available2020-05-08T18:06:04Z-
dc.date.accessioned2020-05-08T18:06:04Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-05-08T18:06:04Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:17:17Z : No. of bitstreams: 1 000493011000007.pdf: 1369883 bytes, checksum: 70a7c4b239c99458b6876b700e7a8914 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-08T18:06:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/340460-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução, Microbiologia e Imunologiapt_BR
dc.contributor.departmentDepartamento de Genética, Evolução e Bioagentespt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordLong non-coding RNAspt_BR
dc.subject.keywordMachine learningpt_BR
dc.subject.keywordTranscriptomept_BR
dc.identifier.source000493011000007pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0001-7442-3338pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-5474-2830pt_BR
dc.creator.orcid0000-0001-5034-4088pt_BR
dc.type.formArtigopt_BR
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