Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/340459
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCamara, Henrique-
dc.contributor.authorunicampSalgueiro, Willian Goulart-
dc.contributor.authorunicampMoro, Raíssa de Paula-
dc.contributor.authorunicampKnittel, Thiago Leite-
dc.contributor.authorunicampSilva, Guilherme Tonon da-
dc.contributor.authorunicampSilva, Silas Pinto da-
dc.contributor.authorunicampMassirer, Katlin Brauer-
dc.contributor.authorunicampMori, Marcelo Alves-
dc.typeArtigopt_BR
dc.titleRNA interference may result in unexpected phenotypes in caenorhabditis eleganspt_BR
dc.contributor.authorDe-Souza, Evandro A.-
dc.contributor.authorCamara, Henrique-
dc.contributor.authorSalgueiro, Willian G.-
dc.contributor.authorMoro, Raissa P.-
dc.contributor.authorKnittel, Thiago L.-
dc.contributor.authorTonon, Guilherme-
dc.contributor.authorPinto, Silas-
dc.contributor.authorPinca, Ana Paula F.-
dc.contributor.authorAntebi, Adam-
dc.contributor.authorPasquinelli, Amy E.-
dc.contributor.authorMassirer, Katlin B.-
dc.contributor.authorMori, Marcelo A.-
dc.subjectRNApt_BR
dc.subject.otherlanguageRNApt_BR
dc.description.abstractRNA interference (RNAi) is a valuable technique to determine gene function. In Caenorhabditis elegans, RNAi can be achieved by feeding worms bacteria carrying a plasmid expressing double-stranded RNA (dsRNA) targeting a gene of interest. The most commonly used plasmid vector for this purpose is L4440. However, it has been noticed that sequences within L4440 may elicit unspecific effects. Here, we provide a comprehensive characterization of these effects and their mechanisms and describe new unexpected phenotypes uncovered by the administration of unspecific exogenous dsRNA. An example involves dsRNA produced by the multiple cloning site (MCS) of L4440, which shares complementary sequences with some widely used reporter vectors and induces partial transgene silencing via the canonical and antiviral RNAi pathway. Going beyond transgene silencing, we found that the reduced embryonic viability of mir-35-41(gk262) mutants is partially reversed by exogenous dsRNA via a mechanism that involves canonical RNAi. These results indicate cross-regulation between different small RNA pathways in C. elegans to regulate embryonic viability. Recognition of the possible unspecific effects elicited by RNAi vectors is important for rigorous interpretation of results from RNAi-based experimentspt_BR
dc.relation.ispartofNucleic acids researchpt_BR
dc.relation.ispartofabbreviationNucleic acids res.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherOxford University Presspt_BR
dc.date.issued2019-
dc.date.monthofcirculationMaypt_BR
dc.language.isoengpt_BR
dc.description.volume47pt_BR
dc.description.issuenumber8pt_BR
dc.description.firstpage3957pt_BR
dc.description.lastpage3969pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0305-1048pt_BR
dc.identifier.eissn1362-4962pt_BR
dc.identifier.doi10.1093/nar/gkz154pt_BR
dc.identifier.urlhttps://academic.oup.com/nar/article/47/8/3957/5369937pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFINANCIADORA DE ESTUDOS E PROJETOS - FINEPpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber444424/2014-8pt_BR
dc.description.sponsordocumentnumbernão tempt_BR
dc.description.sponsordocumentnumber2017/01184-9; 2014/10814-8; 2016/02207-0; 2015/01316-7; 2010/52557-0; 2017/08829-5; 2017/22057-5; 2017/03423-0; 2017/04377-2; 2017/01339-2; 2016/15958-3; 2014/25068-0; 2012/00195-3pt_BR
dc.date.available2020-05-08T18:02:09Z-
dc.date.accessioned2020-05-08T18:02:09Z-
dc.description.provenanceSubmitted by Mariana Aparecida Azevedo (mary1@unicamp.br) on 2020-05-08T18:02:09Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:16:33Z : No. of bitstreams: 1 000473754300017.pdf: 4632733 bytes, checksum: 51d5e354a64285489d16d578622251e0 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-08T18:02:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/340459-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeCentro de Biologia Molecular e Engenharia Genéticapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000473754300017pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-5283-634Xpt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0001-5716-2033vpt_BR
dc.creator.orcid000-0003-3222-9654pt_BR
dc.creator.orcid0000-0003-0894-8915pt_BR
dc.creator.orcid0000-0001-6390-2560pt_BR
dc.creator.orcid0000-0001-7112-5263pt_BR
dc.type.formArtigopt_BR
dc.description.sponsorNoteFundação de Amparo à Pesquisa do Estado de São Paulo [2017/01184-9, 2014/10814-8, 2016/02207-0, 2015/01316-7, 2010/52557-0, 2017/08829-5, 2017/22057-5, 2017/03423-0, 2017/04377-2, 2017/01339-2, 2016/15958-3 and 2014/25068-0 to M.A.M.; 2012/00195-3 to K.B.M.]; Conselho Nacional de Desenvolvimento Científico e Tecnológico [444424/2014-8 to M.A.M.]; Financiadora de Estudos e Projetos [UNIFESP-RPMI PROINFRA 01/2011]; National Institutes of Health [R35 GM127012 to A.E.P.]. Funding for open access charge: Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
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