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|Title:||Forever panting and forever growing : physiology ofsaccharomyces cerevisiae at extremely low oxygen availability in the absence of ergosterol and unsaturated fatty acids|
|Author:||Vale da Costa, Bruno Labate|
Mercier Franco, Luis Fernando
Chaves Filho, Adriano de Britto
Yoshinaga, Marcos Yukio
Basso, Thiago Olitta
Gombert, Andreas Karoly
|Abstract:||We sought to investigate how far the growth of Saccharomyces cerevisiae under full anaerobiosis is dependent on the widely used anaerobic growth factors (AGF) ergosterol and oleic acid. A continuous cultivation setup was employed and, even forcing ultrapure N-2 gas through an O-2 trap upstream of the bioreactor, neither cells from S. cerevisiae CEN.PK113-7D (a lab strain) nor from PE-2 (an industrial strain) washed out after an aerobic-to-anaerobic switch in the absence of AGF. S. cerevisiae PE-2 seemed to cope better than the laboratory strain with this extremely low O-2 availability, since it presented higher biomass yield, lower specific rates of glucose consumption and CO2 formation, and higher survival at low pH. Lipid (fatty acid and sterol) composition dramatically altered when cells were grown anaerobically without AGF: saturated fatty acid, squalene and lanosterol contents increased, when compared to either cells grown aerobically or anaerobically with AGF. We concluded that these lipid alterations negatively affect cell viability during exposure to low pH or high ethanol titers|
|Editor:||Oxford University Press|
|Appears in Collections:||FEA - Artigos e Outros Documentos|
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