Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/340344
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampFranchin, Marcelo-
dc.typeArtigopt_BR
dc.titleNeutrophil extracellular traps (NETs) exacerbate severity of infant sepsispt_BR
dc.contributor.authorColón, David F.-
dc.contributor.authorWanderley, Carlos W.-
dc.contributor.authorFranchin, Marcelo-
dc.contributor.authorSilva, Camila M.-
dc.contributor.authorHiroki, Carlos H.-
dc.contributor.authorCastanheira, Fernanda V. S.-
dc.contributor.authorDonate, Paula B.-
dc.contributor.authorLopes, Alexandre H.-
dc.contributor.authorVolpon, Leila C.-
dc.contributor.authorKavaguti, Silvia K.-
dc.contributor.authorBorges, Vanessa F.-
dc.contributor.authorSpeck-Hernandez, Cesar A.-
dc.contributor.authorRamalho, Fernando-
dc.contributor.authorCarlotti, Ana P.-
dc.contributor.authorCarmona, Fabio-
dc.contributor.authorAlves-Filho, Jose C.-
dc.contributor.authorLiew, Foo Y.-
dc.contributor.authorCunha, Fernando Q.-
dc.subjectSepsept_BR
dc.subjectSuscetibilidadept_BR
dc.subjectArmadilhas extracelularespt_BR
dc.subjectPeptidil arginina desiminase 4pt_BR
dc.subject.otherlanguageSepsispt_BR
dc.subject.otherlanguageSusceptibilidadept_BR
dc.subject.otherlanguageExtracellular trapspt_BR
dc.subject.otherlanguageProtein-arginine deiminase type 4pt_BR
dc.description.abstractNeutrophil extracellular traps (NETs) are innate defense mechanisms that are also implicated in the pathogenesis of organ dysfunction. However, the role of NETs in pediatric sepsis is unknown. Infant (2 weeks old) and adult (6 weeks old) mice were submitted to sepsis by intraperitoneal (i.p.) injection of bacteria suspension or lipopolysaccharide (LPS). Neutrophil infiltration, bacteremia, organ injury, and concentrations of cytokine, NETs, and DNase in the plasma were measured. Production of reactive oxygen and nitrogen species and release of NETs by neutrophils were also evaluated. To investigate the functional role of NETs, mice undergoing sepsis were treated with antibiotic plus rhDNase and the survival, organ injury, and levels of inflammatory markers and NETs were determined. Blood samples from pediatric and adult sepsis patients were collected and the concentrations of NETs measured. Infant C57BL/6 mice subjected to sepsis or LPS-induced endotoxemia produced significantly higher levels of NETs than the adult mice. Moreover, compared to that of the adult mice, this outcome was accompanied by increased organ injury and production of inflammatory cytokines. The increased NETs were associated with elevated expression of Padi4 and histone H3 citrullination in the neutrophils. Furthermore, treatment of infant septic mice with rhDNase or a PAD-4 inhibitor markedly attenuated sepsis. Importantly, pediatric septic patients had high levels of NETs, and the severity of pediatric sepsis was positively correlated with the level of NETs. This study reveals a hitherto unrecognized mechanism of pediatric sepsis susceptibility and suggests that NETs represents a potential target to improve clinical outcomes of sepsispt_BR
dc.relation.ispartofCritical carept_BR
dc.publisher.cityLondonpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherSpringer Naturept_BR
dc.date.issued2019-12-
dc.date.monthofcirculationDec.pt_BR
dc.language.isoengpt_BR
dc.description.volume23pt_BR
dc.description.issuenumber1pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1466-609Xpt_BR
dc.identifier.doi10.1186/s13054-019-2407-8pt_BR
dc.identifier.urlhttps://ccforum.biomedcentral.com/articles/10.1186/s13054-019-2407-8pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumber2013/08216-2pt_BR
dc.date.available2020-05-07T17:29:11Z-
dc.date.accessioned2020-05-07T17:29:11Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-05-07T17:29:11Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:16:14Z : No. of bitstreams: 1 000464166400002.pdf: 4019366 bytes, checksum: b7cabd330d19282deb696ca7fb294158 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-07T17:29:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/340344-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordInfantpt_BR
dc.identifier.source000464166400002pt_BR
dc.creator.orcid0000-0001-5613-2756pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.description.sponsorNoteThe research leading to these results received funding from the São Paulo Research Foundation (FAPESP) under grant agreement n.° 2013/08216-2, the Center for Research in Inflammatory Disease (CRID) under grant agreement 2011/19670-0, the University of São Paulo NAP-DIN (11.1.21625.01.0), the Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico, CNPq) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
Appears in Collections:FOP - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000464166400002.pdf3.93 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.