Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/340339
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampSouza, Ancelmo Rabelo-
dc.contributor.authorunicampYamin, Marriam-
dc.contributor.authorunicampGatti, Maria Silvia Viccari-
dc.contributor.authorunicampBonafé, Carlos Francisco Sampaio-
dc.contributor.authorunicampLima Neto, Daniel Ferreira-
dc.typeArtigopt_BR
dc.titlePorcine parvovirus VP1/VP2 on a time series epitope mapping : exploring the effects of high hydrostatic pressure on the immune recognition of antigenspt_BR
dc.contributor.authorSouza, Ancelmo Rabelo de-
dc.contributor.authorYamin, Marriam-
dc.contributor.authorGava, Danielle-
dc.contributor.authorZanella, Janice Reis Ciacci-
dc.contributor.authorGatti, Maria Sílvia Viccari-
dc.contributor.authorBonafe, Carlos Francisco Sampaio-
dc.contributor.authorLima Neto, Daniel Ferreira de-
dc.subjectMapeamento de epitopospt_BR
dc.subjectParvovirus suínopt_BR
dc.subject.otherlanguageEpitope Mappingpt_BR
dc.subject.otherlanguageParvovirus, Porcinept_BR
dc.description.abstractPorcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for developing new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognizedpt_BR
dc.relation.ispartofVirology journalpt_BR
dc.relation.ispartofabbreviationVirol jpt_BR
dc.publisher.cityLondonpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherSpringer Naturept_BR
dc.date.issued2019-12-
dc.date.monthofcirculationDec.pt_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber1pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1743-422Xpt_BR
dc.identifier.doi10.1186/s12985-019-1165-1pt_BR
dc.identifier.urlhttps://virologyj.biomedcentral.com/track/pdf/10.1186/s12985-019-1165-1pt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2012/1143817pt_BR
dc.description.sponsordocumentnumber2008/09835–0pt_BR
dc.date.available2020-05-07T16:43:28Z-
dc.date.accessioned2020-05-07T16:43:28Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-05-07T16:43:28Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:16:26Z : No. of bitstreams: 1 000470254600003.pdf: 2756357 bytes, checksum: 58b34956991becf13c45beaedca43b30 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-07T16:43:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/340339-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução e Bioagentespt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordEpitope predictionpt_BR
dc.subject.keywordSpot synthesispt_BR
dc.identifier.source000470254600003pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-3614-5503pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigo de pesquisapt_BR
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