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Type: Artigo
Title: Porcine parvovirus VP1/VP2 on a time series epitope mapping : exploring the effects of high hydrostatic pressure on the immune recognition of antigens
Author: Souza, Ancelmo Rabelo de
Yamin, Marriam
Gava, Danielle
Zanella, Janice Reis Ciacci
Gatti, Maria Sílvia Viccari
Bonafe, Carlos Francisco Sampaio
Lima Neto, Daniel Ferreira de
Abstract: Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for developing new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1 h) at − 18 °C, and was compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The conditions tested elicited antibodies against the VP1/VP2 antigen that differed in relation to the response time and the profile of structurally available regions that were recognized
Subject: Mapeamento de epitopos
Parvovirus suíno
Country: Reino Unido
Editor: Springer Nature
Rights: Aberto
Identifier DOI: 10.1186/s12985-019-1165-1
Date Issue: Dec-2019
Appears in Collections:IB - Artigos e Outros Documentos

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