Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/340247
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCrucello, Aline-
dc.contributor.authorunicampFerreira Filho, Jaire Alves-
dc.contributor.authorunicampSant'Ana, Anderson de Souza-
dc.typeArtigopt_BR
dc.titleCharacterization of novel small RNAs (sRNAs) contributing to the desiccation response of Salmonella enterica serovar Typhimuriumpt_BR
dc.contributor.authorBarnhill, Emmaline C.-
dc.contributor.authorCrucello, Aline-
dc.contributor.authorHouserova, Dominika-
dc.contributor.authorKing, Valeria M.-
dc.contributor.authorAmin, Shivam V.-
dc.contributor.authorRoberts, Justin T.-
dc.contributor.authorZambrano, Michael E.-
dc.contributor.authorDeMeis, Jeffrey D.-
dc.contributor.authorDahmer, Donavon J.-
dc.contributor.authorIjaz, Zara-
dc.contributor.authorBarchie, Addison A.-
dc.contributor.authorWatters, Brianna C.-
dc.contributor.authorPrusak, James E.-
dc.contributor.authorDean, Meghan A.-
dc.contributor.authorHolton, Nathaniel W.-
dc.contributor.authorFerreira-Filho, Jaire A.-
dc.contributor.authorSant'Ana, Anderson S.-
dc.contributor.authorSpector, Michael P.-
dc.contributor.authorBorchert, Glen M.-
dc.subjectBactériaspt_BR
dc.subject.otherlanguageBacteriapt_BR
dc.description.abstractNoncoding RNA (ncRNA) modulation of gene expression has now been ubiquitously observed across all domains of life. An increasingly apparent role of ncRNAs is to coordinate changes in gene expressions in response to environmental stress. Salmonella enterica, a common food-born pathogen, is known for its striking ability to survive, adapt, and thrive in various unfavourable environments which makes it a particularly difficult pathogen to eliminate as well as an interesting model in which to study ncRNA contributions to cellular stress response. Mounting evidence now suggests that small RNAs (sRNAs) represent key regulators of Salmonella stress adaptation. Approximately 50-500 nucleotides in length, sRNAs regulate gene expression through complementary base pairing with molecular targets and have recently been suggested to outnumber protein-coding genes in bacteria. In this work, we employ small RNA transcriptome sequencing to characterize changes in the sRNA profiles of Salmonella in response to desiccation. In all, we identify 102 previously annotated sRNAs significantly differentially expressed during desiccation; and excitingly, 71 novel sRNAs likewise differentially expressed. Small transcript northern blotting and qRT-PCRs confirm the identities and expressions of several of our novel sRNAs, and computational analyses indicate the majority are highly conserved and structurally related to characterized sRNAs. Predicted sRNA targets include several proteins necessary for desiccation survival and this, in part, suggests a role for desiccation-regulated sRNAs in this stress response. Furthermore, we find individual knock-outs of two of the novel sRNAs identified herein, either sRNA1320429 or sRNA3981754, significantly impairs the ability of Salmonella to survive desiccation, confirming their involvements (and suggesting the potential involvements of other sRNAs we identify in this work) in the Salmonella response to desiccationpt_BR
dc.relation.ispartofRNA biologypt_BR
dc.relation.ispartofabbreviationRNA biol.pt_BR
dc.publisher.cityPhiladelphia, PApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherTaylor & Francispt_BR
dc.date.issued2019-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber11pt_BR
dc.description.firstpage1643pt_BR
dc.description.lastpage1657pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1547-6286pt_BR
dc.identifier.eissn1555-8584pt_BR
dc.identifier.doi10.1080/15476286.2019.1653680pt_BR
dc.identifier.urlhttps://www.tandfonline.com/doi/full/10.1080/15476286.2019.1653680pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber302763/2014-7; 305804/2017-0pt_BR
dc.description.sponsordocumentnumber2014/17387-8; 2015/19400-4pt_BR
dc.date.available2020-05-06T15:44:26Z-
dc.date.accessioned2020-05-06T15:44:26Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-05-06T15:44:26Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-08-27T19:16:49Z : No. of bitstreams: 1 000481303900001.pdf: 5106543 bytes, checksum: 3f4105df19ef92a37d943db2af5edd6b (MD5)en
dc.description.provenanceMade available in DSpace on 2020-05-06T15:44:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/340247-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Ciência de Alimentospt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.subject.keywordDesiccationpt_BR
dc.subject.keywordNcRNApt_BR
dc.subject.keywordNoncoding RNApt_BR
dc.subject.keywordOsmoprotectantpt_BR
dc.subject.keywordProkaryoticpt_BR
dc.subject.keywordProPpt_BR
dc.subject.keywordSalmonellapt_BR
dc.subject.keywordSRNApt_BR
dc.subject.keywordStresspt_BR
dc.subject.keywordTranscriptomept_BR
dc.identifier.source000481303900001pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0001-9690-7704pt_BR
dc.creator.orcid0000-0001-5052-2315pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.description.sponsorNoteFunding was provided in part by NSF CAREER grant 1350064 (GMB) awarded by Division of Molecular and Cellular Biosciences (with cofunding provided by the NSF EPSCoR program). Graduate funding was also provided in part by Alabama Commission on Higher Education ALEPSCoR grants 150380 (JTR),160330 (VMK), and 180435 (DH). Postdoctoral funding was provided by São Paulo Research Foundation (FAPESP) Grants # 2014/17387-8 and # 2015/19400-4 (AC). A.S. Sant’Ana acknowledges the support of ‘Conselho Nacional de Desenvolvimento Cientifico e Tecnológico’ (CNPq) (Grants #302763/2014-7; #305804/2017-0); Alabama Commission on Higher Education [180435]; Alabama Commission on Higher Education [160330]; Alabama Commission on Higher Education [150380]; Conselho Nacional de Desenvolvimento Científico e Tecnológico [305804/2017-0]; Fundo de Apoio ao Ensino, à Pesquisa e Extensão, Universidade Estadual de Campinas [2015/19400-4]; Fundo de Apoio ao Ensino, à Pesquisa e Extensão, Universidade Estadual de Campinas (BR) [2014/17387-8]; Conselho Nacional de Desenvolvimento Científico e Tecnológico (BR) [302763/2014-7]pt_BR
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