Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/340095
Type: Artigo
Title: Stability of immobilized laccase on Luffa Cylindrica fibers and assessment of synthetic hormone degradation
Author: Alves Ribeiro Lacerda, Monike Fabiane
Lopes, Flavio Marques
Sartoratto, Adilson
Ponezi, Alexandre Nunes
Thomaz, Douglas Vieira
Schimidt, Fernando
Santiago, Mariangela Fontes
Abstract: In this work were studied the pH, thermal, and storage stability of free and immobilized laccases. Enzymes were produced by Pleurotus ostreatus on potato dextrose (PD) broth and potato dextrose modified (PDM) broth, and immobilized using Luffa cylindrica fibers as support. Both free and immobilized enzymes were assessed on their respective enzymatic activities and for 17 alpha-ethinylestradiol (EE2) degradation. The optimum pH conditions concerning laccase activity ranged from 3.6 to 4.6, while temperature ranged between 30 degrees C and 50 degrees C for both free and immobilized enzyme. Laccase produced using PD broth presented greater storage stability and thermal stability than that of PDM. Best EE2 removals were of 79.22% and 75.00% for the free and immobilized enzymes, respectively. Removal rates were assessed during 8 h at pH 5. The removal of 17 alpha-ethinylestradiol was stabilized in the fourth cycle of use. Results imply that immobilization promoted stability towards pH and temperature variations, although media played a decisive role in the enzymatic activity. Both free and immobilized laccases of P. ostreatus were able to degrade EE2, whereas immobilized laccase in PDM medium presented possible reuse applicability, albeit removal was not optimal when compared to other reports
Subject: Biorremediação
Country: Estados Unidos
Editor: Taylor & Francis
Rights: Fechado
Identifier DOI: 10.1080/10826068.2018.1525568
Address: https://www.tandfonline.com/doi/full/10.1080/10826068.2018.1525568
Date Issue: 2019
Appears in Collections:CPQBA - Artigos e Outros Documentos

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