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Type: Artigo
Title: Interesterified fats induce deleterious effects on adipose tissue and liver in LDLr-KO mice
Author: Ferrari Lavrador, Maria Silvia
Afonso, Milessa Silva
Cintra, Dennys Esper
Koike, Marcia
Nunes, Valeria Sutti
Demasi, Marina
Lin, Chin Jia
Masuzawa Beda, Lis Mie
Gioielli, Luiz Antonio
Assis Bombo, Renata de Paula
Machado, Roberta Marcondes
Catanozi, Sergio
Nakandakare, Edna Regina
Lottenberg, Ana Maria
Abstract: Interesterified fats are being widely used by the food industry in an attempt to replace trans fatty acids. The effect of interesterified fats containing palmitic or stearic acids on lipid metabolism and inflammatory signaling pathways in adipose and hepatic tissues was evaluated. Male LDLr-KO mice were fed a high-fat diet containing polyunsaturated (PUFA), palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR), or stearic interesterified (STEAR INTER) fats for 16 weeks. The expression of genes and protein levels involved in lipid metabolism and inflammatory processes in liver and white adipose tissue was determined by quantitative RT-PCR and by Western blot, respectively. The infiltration of inflammatory cells in hepatic and adipose tissues was determined by eosin and hematoxylin, while liver collagen content was determined by Sirius Red staining. Both interesterified fats increased liver collagen content and JNK phosphorylation. Additionally, the STEAR INTER group developed nonalcoholic steatohepatitis (NASH) associated with higher neutrophil infiltration. PALM INTER induced adipose tissue expansion and enlargement of adipocytes. Furthermore, PALM INTER triggered increased IKK phosphorylation and TNF protein content, conditions associated with the upstream activation of the NFkB signaling pathway. STEAR INTER induced NASH, while PALM INTER triggered hepatic fibrosis and adipocyte hypertrophy with inflammatory response in LDLr-KO mice
Subject: Obesidade
Country: Suiça
Editor: MDPI
Rights: Aberto
Identifier DOI: 10.3390/nu11020466
Date Issue: 2019
Appears in Collections:FCA - Artigos e Outros Documentos

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