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Type: Artigo
Title: In vitro immunotoxicological assessment of a potent microbicidal nanocomposite based on graphene oxide and silver nanoparticles
Author: Visani de Luna, Luis Augusto
Zorgi, Nahiara Esteves
Mazarin de Moraes, Ana Carolina
da Silva, Douglas Soares
Consonni, Silvio Roberto
Giorgio, Selma
Alves, Oswaldo Luiz
Abstract: Graphene oxide (GO) and silver nanoparticles (AgNPs) can be formed into a hybrid nanomaterial, known as GOAg nanocomposite, which presents high antibacterial activity. The successful translation of this nanomaterial into medical use depends on critical information about its toxicological profile. In keeping with a Safe-by-design approach, we evaluated the immunotoxicity of GOAg using J774 and primary murine macrophages. The interaction between GOAg and macrophages was investigated with a scanning electron microscope (SEM). High-throughput technologies were employed to evaluate cell viability, apoptosis/necrosis, mitochondrial depolarization and lipid peroxidation. The inflammogenicity of nanomaterials was predicted after quantification of the cytokines IL-1 beta, TNF-alpha and IL-10 before and after stimulation with interferon-gamma (IFN-gamma). The ratio between CD80 and CD206 macrophage populations were also estimated. In addition, the production of nitric oxide (NO) was investigated. SEM surveys revealed the potential of GOAg to induce frustrated phagocytosis. GOAg induced a dose-dependent mitochondrial depolarization, apoptosis and lipid peroxidation to J774 macrophages. GOAg toxicity was not modified in an inflammatory microenvironment, but its toxicity was within the range of concentrations used in bacterial inactivation. GOAg did not induce primary macrophages to significantly produce inflammatory cytokines, and previous macrophage stimulation did not enhance GOAg inflammogenicity. Additionally, the pristine nanomaterials and GOAg do not shift macrophages polarization towards M1. Sublethal concentrations of GOAg did not impair macrophages NO production. Finally, we suggest options for improvement of GOAg nanocomposite in ways that may help minimize its possible adverse outcomes to human health
Subject: Nanopartículas de prata
Country: Reino Unido
Editor: Taylor & Francis Online
Rights: Fechado
Identifier DOI: 10.1080/17435390.2018.1537410
Date Issue: 2019
Appears in Collections:IQ - Artigos e Outros Documentos
IB - Artigos e Outros Documentos

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