Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/337462
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCabral, Lucélia-
dc.contributor.authorunicampNoronha, Melline Fontes-
dc.contributor.authorunicampSousa, Sanderson Tarciso Pereira de-
dc.contributor.authorunicampLacerda Júnior, Gileno Vieira-
dc.contributor.authorunicampRichter, Larissa-
dc.contributor.authorunicampFostier, Anne Hélène-
dc.contributor.authorunicampOliveira, Valeria Maia de-
dc.typeArtigopt_BR
dc.titleThe metagenomic landscape of xenobiotics biodegradation in mangrove sedimentspt_BR
dc.contributor.authorCabral, Lucelia-
dc.contributor.authorNoronha, Melline Fontes-
dc.contributor.authorPereira de Sousa, Sanderson Tarciso-
dc.contributor.authorLacerda-Junior, Gileno Vieira-
dc.contributor.authorRichter, Larissa-
dc.contributor.authorFostier, Anne Helene-
dc.contributor.authorAndreote, Fernando Dini-
dc.contributor.authorHess, Matthias-
dc.contributor.authorde Oliveira, Valeria Maia-
dc.subjectMetagenômicapt_BR
dc.subjectXenobióticospt_BR
dc.subject.otherlanguageMetagenomicspt_BR
dc.subject.otherlanguageXenobioticspt_BR
dc.description.abstractMetagenomics is a powerful approach to study microorganisms present in any given environment and their potential to maintain and improve ecosystem health without the need of cultivating these microorganisms in the laboratory. In this study, we combined a cultivation-independent metagenomics approach with functional assays to identify the detoxification potential of microbial genes evaluating their potential to contribute to xenobiotics resistance in oil-impacted mangrove sediments. A metagenomic fosmid library containing 12,960 clones from highly contaminated mangrove sediment was used in this study. For assessment of metal resistance, clones were grown in culture medium with increasing concentrations of mercury. The analyses metagenomic library sequences revealed the presence of genes related to heavy metals and antibiotics resistance in the oil-impacted mangrove microbiome. The taxonomic profiling of these sequences suggests that at the genus level, Geobacter was the most abundant genus in our dataset. A functional screening assessment of the metagenomic library successfully detected 24 potential heavy metal tolerant clones, six of which were capable of growing with increased concentrations of mercury. The genetic characterization of selected clones allowed the detection of genes related to detoxification processes, such as chromate transport protein ChrA, haloacid dehalogenase-like hydrolase, lipopolysaccharide transport system, and 3-oxoacyl-[acyl-carrier-protein] reductase. Clones were capable of growing in medium containing increased concentrations of metals and antibiotics, but none manifested strong mercury removal from culture medium characteristic of mercuric reductase activity. These results suggest that resistance to xenobiotic stress varies greatly and that additional studies to elucidate the potential of metal biotransformation need to be carried out with the goal of improving bioremediation applicationpt_BR
dc.relation.ispartofEcotoxicology and environmental safetypt_BR
dc.relation.ispartofabbreviationEcotoxicol. environ. saf.pt_BR
dc.publisher.cityMaryland Heights, MOpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2019-
dc.date.monthofcirculationSep.pt_BR
dc.language.isoengpt_BR
dc.description.volume179pt_BR
dc.description.firstpage232pt_BR
dc.description.lastpage240pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0147-6513pt_BR
dc.identifier.eissn1090-2414pt_BR
dc.identifier.doi10.1016/j.ecoenv.2019.04.044pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0147651319304774pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2012/16850-0; 2013/20670-0; 2012/06245-2; 2011/50809-5pt_BR
dc.date.available2020-03-26T15:34:26Z-
dc.date.accessioned2020-03-26T15:34:26Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2020-03-26T15:34:26Z No. of bitstreams: 0. Added 1 bitstream(s) on 2020-07-20T14:17:49Z : No. of bitstreams: 1 000469161200028.pdf: 1526685 bytes, checksum: ac0c37641ba490523352bea74861058a (MD5)en
dc.description.provenanceMade available in DSpace on 2020-03-26T15:34:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2019en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/337462-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Química Analíticapt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeCentro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolaspt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.subject.keywordResistomept_BR
dc.subject.keywordFunctional screeningpt_BR
dc.subject.keywordOil-impacted sedimentspt_BR
dc.identifier.source000469161200028pt_BR
dc.creator.orcid0000-0003-1743-6364pt_BR
dc.creator.orcid0000-0003-0914-4679pt_BR
dc.creator.orcid0000-0002-3928-240Xpt_BR
dc.creator.orcid0000-0003-4493-0755pt_BR
dc.creator.orcid0000-0002-7438-7338pt_BR
dc.creator.orcid0000-0002-6250-7419pt_BR
dc.creator.orcid0000-0002-2436-834Xpt_BR
dc.type.formArtigopt_BR
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