Please use this identifier to cite or link to this item:
Type: Artigo
Title: Abnormal brown adipose tissue mitochondrial structure and function in IL10 deficiency
Author: Lima-Júnior, José C. de
Souza, Gabriela F.
Moura-Assis, Alexandre
Gaspar, Rodrigo S.
Gaspar, Joana M.
Rocha, Andréa L.
Ferrucci, Danilo L.
Lima, Tanes I.
Victório, Sheila C.
Bonfante, Ivan L.P.
Cavaglieri, Claudia R.
Pareja, José C.
Brunetto, Sérgio Q.
Ramos, Celso D.
Mori, Marcelo A.
Silveira, Leonardo R.
Segundo, Gesmar R.S.
Velloso, Lício A.
Abstract: Inflammation is the most relevant mechanism linking obesity with insulin-resistance and metabolic disease. It impacts the structure and function of tissues and organs involved in metabolism, such as the liver, pancreatic islets and the hypothalamus. Brown adipose tissue has emerged as an important component of whole body energy homeostasis, controlling caloric expenditure through the regulation of non-shivering thermogenesis. However, little is known about the impact of systemic inflammation on the structure and function of brown adipose tissue. The relations between IL10 and mitochondria structure/function and also with thermogenesis were evaluated by bioinformatics using human and rodent data. Real-time PCR, immunoblot, fluorescence and transmission electron microscopy were employed to determine the effect of IL10 in the brown adipose tissue of wild type and IL10 knockout mice. IL10 knockout mice, a model of systemic inflammation, present severe structural abnormalities of brown adipose tissue mitochondria, which are round-shaped with loss of cristae structure and increased fragmentation. IL10 deficiency leads to newborn cold intolerance and impaired UCP1-dependent brown adipose tissue mitochondrial respiration. The reduction of systemic inflammation with an anti-TNFα monoclonal antibody partially rescued the structural but not the functional abnormalities of brown adipose tissue mitochondria. Using bioinformatics analyses we show that in both humans and mice, IL10 transcripts correlate with mitochondrial lipid metabolism and caspase gene expression. IL10 and systemic inflammation play a central role in the regulation of brown adipose tissue by controlling mitochondrial structure and function
metadata.dc.description.abstractalternative: Obesity
Subject: Obesidade
Country: Países Baixos
Editor: Elsevier
Rights: Aberto
Identifier DOI: 10.1016/j.ebiom.2018.11.041
Date Issue: Jan-2019
Appears in Collections:IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos
FEF - Artigos e Outros Documentos
CEB - Artigos e Outros Documentos
FCA - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000456677400051.pdf4.03 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.