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Type: Artigo
Title: Gene duplication in the sugarcane genome: a case study of allele interactions and evolutionary patterns in two genic regions
Author: Sforca, Danilo Augusto
Vautrin, Sonia
Cardoso-Silva, Claudio Benicio
Mancini, Melina Cristina
Romero-da Cruz, Maria Victoria
Pereira, Guilherme da Silva
Conte, Monica
Bellec, Arnaud
Dahmer, Nair
Fourment, Joelle
Rodde, Nathalie
Van Sluys, Marie-Anne
Vicentini, Renato
Franco Garcia, Antonio Augusto
Forni-Martins, Eliana Regina
Carneiro, Monalisa Sampaio
Hoffmann, Hermann Paulo
Pinto, Luciana Rossini
de Andrade Landell, Marcos Guimaraes
Vincentz, Michel
Berges, Helene
de Souza, Anete Pereira
Abstract: Sugarcane (Saccharum spp.) is highly polyploid and aneuploid. Modern cultivars are derived from hybridization between S. officinarum and S. spontaneum. This combination results in a genome exhibiting variable ploidy among different loci, a huge genome size (similar to 10 Gb) and a high content of repetitive regions. An approach using genomic, transcriptomic, and genetic mapping can improve our knowledge of the behavior of genetics in sugarcane. The hypothetical HP600 and Centromere Protein C (CENP-C) genes from sugarcane were used to elucidate the allelic expression and genomic and genetic behaviors of this complex polyploid. The physically linked side-by-side genes HP600 and CENP-C were found in two different homeologous chromosome groups with ploidies of eight and ten. The first region (Region01) was a Sorghum bicolor ortholog region with all haplotypes of HP600 and CENP-C expressed, but HP600 exhibited an unbalanced haplotype expression. The second region (Region02) was a scrambled sugarcane sequence formed from different noncollinear genes containing partial duplications of HP600 and CENP-C (paralogs). This duplication resulted in a non-expressed HP600 pseudogene and a recombined fusion version of CENP-C and the orthologous gene Sobic. 003G299500 with at least two chimeric gene haplotypes expressed. It was also determined that it occurred before Saccharum genus formation and after the separation of sorghum and sugarcane. A linkage map was constructed using markers from nonduplicated Region01 and for the duplication (Region01 and Region02). We compare the physical and linkage maps, demonstrating the possibility of mapping markers located in duplicated regions with markers in nonduplicated region. Our results contribute directly to the improvement of linkage mapping in complex polyploids and improve the integration of physical and genetic data for sugarcane breeding programs. Thus, we describe the complexity involved in sugarcane genetics and genomics and allelic dynamics, which can be useful for understanding complex polyploid genomes
Subject: Cana-de-açucar
Country: Suiça
Editor: Frontiers
Rights: Aberto
Identifier DOI: 10.3389/fpls.2019.00553
Date Issue: 2019
Appears in Collections:IB - Artigos e Outros Documentos
CBMEG - Artigos e Outros Documentos

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