Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/337277
Type: Artigo
Title: Grape peel powder promotes intestinal barrier homeostasis in acute TNBS-colitis: a major role for dietary fiber and fiber-bound polyphenols
Author: Maurer, Luana Haselein
Betim Cazarin, Cinthia Bau
Quatrin, Andreia
Minuzzi, Natalia Machado
Costa, Eduarda Lasch
Morari, Joseane
Velloso, Lido Augusto
Leal, Raquel Franco
Rodrigues, Eliseu
Bochi, Vivian Caetano
Marstica Junior, Mario Roberto
Emanuelli, Tatiana
Abstract: Inflammatory bowel diseases are characterized by impaired intestinal barrier function. This study aimed to evaluate the effects of grape peel powder (GPP) and its bioactive rich-fractions on the barrier function and colonic injury in a model of colitis induced by 2,4,6 trinitrobenzene sulfonic acid (TNBS). Wistar rats received diets supplemented with either GPP (8%), extractable polyphenols (EP), non-extractable polyphenols-rich fraction (NEP-F), or polyphenols-poor, fiber-rich fraction (F) from grapes at amounts equivalent to the GPP group during 15 days before and for 7 days after colitis induction. NEP-F has decreased the extension of colonic lesion but the other grape peel bioactive fractions did not protect against macroscopic or microscopic colonic damage, EP diet increased macroscopic colonic damage. GPP, EP, and NEP-F reduced claudin-2 mRNA expression, whereas GPP and F fraction increased occludin and ZO-1 mRNA expression. All experimental diets reduced the colitis-triggered increase of MMP-9 mRNA expression. Colitis reduced by 30% the production of cecal short-chain fatty acids (SCFA). GPP and NEP-F completely protected against this effect, whereas F fraction was ineffective. Only GPP and NEP-F were able to decrease the upregulation of GRP94 mRNA triggered by colitis. Dietary fiber seems to reestablish the intestinal barrier function, whereas fiber-bound phenolics were able to restore cecal metabolism to produce beneficial metabolites like SCFA and to reduce the activation of the unfolded protein response.
Subject: Fibras na nutrição humana
Inflamação
Uva
Country: Reino Unido
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.foodres.2019.04.068
Address: https://www.sciencedirect.com/science/article/pii/S096399691930300X
Date Issue: 2019
Appears in Collections:FCM - Artigos e Outros Documentos
FEA - Artigos e Outros Documentos

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