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Type: Artigo
Title: Molecular eco-epidemiology on the sympatric Chagas disease vectors triatoma brasiliensis and triatoma petrocchiae: ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping
Author: Lima-Oliveira, Tarcianne M.
Fontes, Fernanda von Hertwig M.
Lilioso, Mauricio
Pires-Silva, Dayane
Geraldes Teixeira, Marta Maria
Vergara Meza, Jose Gabriel
Harry, Myriam
Filee, Jonathan
Costa, Jane
Valenca-Barbosa, Carolina
Folly-Ramos, Elaine
Almeida, Carlos Eduardo
Abstract: Triatoma petrocchiae is the newly member of the Triatoma brasiliensis species complex. This species overlaps with T. brasiliensis in geographic and ecotypic occupation in the sylvatic habitat because both inhabit rocky outcrops in the semi-arid portion of Brazilian northeast. In this region T. brasiliensis is the most important Chagas disease vector because it constantly colonizes domiciles. In contrast, T. petrocchiae is rarely found in peri or intradomiciliary habitats - reason why little is known about this species. Therefore, Here, we present information for the first time on. the T. petrocchiae ecotopes, genetic diversity, Trypanosoma cruzi prevalence/genotyping in comparison to T. brasiliensis. We found T. brasilensis (N=223) and T. petrocchiae (N=69) in co-habitation in rocky outcrops in three Districts of Paraiba and Rio Grande do Norte states. Forty-tree T. petrocchiae insects of eleven sampling spots (composing three geographic populations) were genotyped for the mitochondrial Cyt B gene and little geographic structure was observed. Tajima's D test suggested that species is evolving toward a mutation-drift equilibrium in our collection range. Sylvatic T. petrocchiae had 4% (3/68) of infected insects by T. cruzi, whereas T. brasiliensis had 26% (59/223). Fluorescent Fragment Length Barcoding demonstrated that all three T. petrocchiae harbored TcI whereas T. brasiliensis had TcI, but also TcIII, TcII/TcVI and T. rangeli genotype A, sometimes under mixed infections. None of infected T. petrocchiae were carrying mixed infections. However, this result should be confirmed using a larger pool of infected bugs. We here presented the first documentation of T. rangeli infecting T. brasiliensis. The finding of infected T. petrocchiae calls for constant vector monitoring because the epidemiologic scenario is dynamic and sylvatic vectors are progressively found in adaptation to anthropic environments
Subject: Doença de Chagas
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.actatropica.2019.105188
Date Issue: 2020
Appears in Collections:IB - Artigos e Outros Documentos

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