Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/337107
Type: Artigo
Title: Low-frequency ultrasound with short application time improves cellulase activity and reducing sugars release
Author: de Carvalho Silvello, Maria Augusta
Martinez, Julian
Goldbeck, Rosana
Abstract: In this study, we investigated the effect of ultrasound (US) on the activity of commercial cellulase (Celluclast (R) 1.5 L) in the absence and in the presence of a cellulosic substrate (Avicel (R), 2% w.v(-1)) using a central composite rotatable design. Sonication time (10 to 330 s), US intensity (120.6 to 263.7 W cm(-2)), and reaction temperature (25 to 50 degrees C) were varied using a horn-type ultrasound reactor, and endoglucanase (CMCase) and total cellulase (FPase) activities were determined. US intensity had a positive effect on enzyme activity. Under optimal conditions (170 s, 180.8 W cm(-2), and 25 degrees C), CMCase activity was 13% higher than that of the control. In the presence of substrate, CMCase activity increased by 33.87% and K-M reduced by 23% in relation to that of the control. The theoretical yield of cellulose was 42.08%. Cellulase activity can be improved by US treatment to maximize productivity gains and reduce costs in second-generation ethanol production, by the action of a low-frequency ultrasound with a short ultrasonication time of application
Subject: Celulases
Cinética de enzimas
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s12010-019-03148-1
Address: https://link.springer.com/article/10.1007%2Fs12010-019-03148-1
Date Issue: 2020
Appears in Collections:FEA - Artigos e Outros Documentos

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