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Type: Artigo
Title: MicroRNA profile of pediatric pilocytic astrocytomas identifies two tumor-specific signatures when compared to non-neoplastic white matter
Author: Darrigo Junior, Luiz Guilherme
Peixoto Lira, Regia Caroline
Fedatto, Paola Fernanda
Marco Antonio, David Santos
Valera, Elvis Terci
Aguiar, Simone
Yunes, Jose Andres
Brandalise, Silvia Regina
Neder, Luciano
Saggioro, Fabiano Pinto
Becker, Aline Paixao
de Oliveira, Ricardo Santos
Machado, Helio Rubens
Panepucci, Rodrigo Alexandre
Tone, Luiz Gonzaga
Scrideli, Carlos Alberto
Abstract: PurposesPilocytic astrocytoma (PA) is a low-grade neoplasm frequently found in childhood. PA is characterized by slow growth and a relatively good prognosis. Genetic mechanisms such as activation of MAPK, BRAF gene deregulation and neurofibromatosis type 1 (NF1) syndrome have been associated with PA development. Epigenetic signature and miRNA expression profile are providing new insights about different types of tumor, including PAs.MethodsIn the present study we evaluated global miRNA expression in 16 microdissected pediatric PA specimens, three NF1-associated PAs and 11 cerebral white matter (WM) samples by the microarray method. An additional cohort of 20 PAs was used to validate by qRT-PCR the expression of six miRNAs differentially expressed in the microarray data.ResultsUnsupervised hierarchical clustering analysis distinguished one cluster with nine PAs, including all NF1 cases and a second group consisting of the WM samples and seven PAs. Among 88 differentially expressed miRNAs between PAs and WM samples, the most underexpressed ones regulate classical pathways of tumorigenesis, while the most overexpressed miRNAs are related to pathways such as focal adhesion, P53 signaling pathway and gliomagenesis. The PAs/NF1 presented a subset of underexpressed miRNAs, which was also associated with known deregulated pathways in cancer such as cell cycle and hippo pathway.ConclusionsIn summary, our data demonstrate that PA harbors at least two distinct miRNA signatures, including a subgroup of patients with NF1/PA lesions
Subject: Astrocitoma
Neurofibromatose 1
Country: Estados Unidos
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s11060-018-03042-6
Date Issue: 2019
Appears in Collections:IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos

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