Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/336900
Type: Artigo
Title: Coenzyme Q10 supplementation acts as antioxidant on dystrophic muscle cells
Author: Mizobuti, Daniela Sayuri
Fogaça, Aline Reis
Moraes, Fernanda dos Santos Rapucci
Moraes, Luis Henrique Rapucci
Mâncio, Rafael Dias
Hermes, Túlio de Almeida
Macedo, Aline Barbosa
Valduga, Amanda Harduim
Lourenço, Caroline Caramano de
Pereira, Elaine Cristina Leite
Minatel, Elaine
Abstract: Increased oxidative stress is a frequent feature in Duchenne muscular dystrophy (DMD). High reactive oxygen species (ROS) levels, associated with altered enzyme antioxidant activity, have been reported in dystrophic patients and mdx mice, an experimental model of DMD. In this study, we investigated the effects of coenzyme Q10 (CoQ10) on oxidative stress marker levels and calcium concentration in primary cultures of dystrophic muscle cells from mdx mice. Primary cultures of skeletal muscle cells from C57BL/10 and mdx mice were treated with coenzyme Q10 (5 μM) for 24 h. The untreated mdx and C57BL/10 muscle cells were used as controls. The MTT and live/dead cell assays showed that CoQ10 presented no cytotoxic effect on normal and dystrophic muscle cells. Intracellular calcium concentration, H2O2 production, 4-HNE, and SOD-2 levels were higher in mdx muscle cells. No significant difference in the catalase, GPx, and Gr levels was found between experimental groups. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. This study demonstrated that CoQ10 treatment was able to reduce levels of oxidative stress markers, such as H2O2, acting as an antioxidant, as well as decreasing abnormal intracellular calcium influx in dystrophic muscles cells. Our findings also suggest that the decrease of oxidative stress reduces the need for upregulation of antioxidant pathways, such as SOD and GSH
Subject: Estresse oxidativo
Células musculares
Country: Países Baixos
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s12192-019-01039-2
Address: https://link.springer.com/article/10.1007/s12192-019-01039-2
Date Issue: Nov-2019
Appears in Collections:IB - Artigos e Outros Documentos

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