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Type: Artigo
Title: ATP synthase subunit beta immunostaining is reduced in the sclerotic hippocampus of epilepsy patients
Author: Boas Mota, Marcelo Vilas
Zaidan, Bruna Cunha
do Canto, Amanda Morato
Ghizoni, Enrico
Tedeschi, Helder
Queiroz, Luciano de Souza
Alvim, Marina K. M.
Cendes, Fernando
Lopes-Cendes, Iscia
Schenka, Andre Almeida
Vieira, Andre Schwambach
Rogerio, Fabio
Abstract: Epilepsy is a common disease presenting with recurrent seizures. Hippocampal sclerosis (HS) is the commonest histopathological alteration in patients with temporal lobe epilepsy (TLE) undergoing surgery. HS physiopathogenesis is debatable. We have recently studied, by using mass spectrometry-based proteomics, an experimental model of TLE induced by electrical stimulation. Specifically, protein expressions of both the beta subunit of mitochondrial ATP synthase (ATP5B) and of membrane ATPases were found to be reduced. Here, we investigated tissue distribution of ATP5B and sodium/potassium-transporting ATPase subunit alpha-3 (NKA3), a protein associated with neuromuscular excitability disorders, in human hippocampi resected en bloc for HS treatment (n=15). We used immunohistochemistry and the stained area was digitally evaluated (increase in binary contrast of microscopic fields) in the hippocampal sectors (CA1-CA4) and dentate gyrus. All HS samples were classified as Type 1, according to the International League Against Epilepsy (ILAE) 2013 Classification (predominant cell loss in CA1 and CA4). ATP5B was significantly decreased in all sectors and dentate gyrus of HS patients compared with individuals submitted to necropsy and without history of neurological alterations (n=10). NKA3 expression showed no difference. Moreover, we identified a negative correlation between frequency of pre-operative seizures and number of neurons in CA1. In conclusion, our data showed similarity between changes in protein expression in a model of TLE and individuals with HS. ATP5B reduction would be at least in part due to neuronal loss. Future investigations on ATP5B activity could provide insights into the process of such cell loss
Subject: Epilepsia
Country: Estados Unidos
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s10571-018-0641-2
Date Issue: 2019
Appears in Collections:FCM - Artigos e Outros Documentos

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