Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/33677
Type: Artigo de periódico
Title: In vitro evaluation of the susceptibility of endodontic pathogens to calcium hydroxide combined with different vehicles
Author: Vianna, Morgana Eli
Gomes, Brenda Paula Figueiredo de Almeida
Sena, Neylla Teixeira
Zaia, Alexandre Augusto
Ferraz, Caio Cezar Randi
Souza Filho, Francisco José de
Abstract: The aim of this study was to investigate in vitro the antimicrobial activity of calcium hydroxide [Ca(OH)2] in combination with different vehicles against endodontic pathogens. For such purpose, a broth dilution test was performed. Pastes were prepared with Ca(OH)2 powder and the following vehicles: sterile water, glycerin, camphorated monochlorophenol (CMCP), CMCP + glycerin, polyethyleneglycol and CMCP + polyethyleneglycol. The time required for the pastes to produce negative cultures against the tested microorganisms was recorded and analyzed statistically using the Kruskal Wallis test at 5% significance level. Timing for pastes to eliminate the aerobic and facultative anaerobic microorganisms ranged from 6 to 24 h, while strict anaerobic microorganisms were inhibited within 30 s to 5 min. Microbial susceptibility, ranked from weakest to strongest, can be presented as follows: Enterococcus faecalis (the most resistant microorganism), Candida albicans, Staphylococcus aureus, Porphyromonas gingivalis, Porphyromonas endodontalis and Prevotella intermedia (the last two microorganisms required the same time to be eliminated). In conclusion, calcium hydroxide pastes needed more time to eliminate facultative than anaereobic microorganisms. These findings suggest that the antimicrobial property is related both to paste formulation and to microbial susceptibility.
O objetivo deste estudo foi avaliar in vitro a atividade antimicrobiana do hidróxido de cálcio [Ca(OH)2] em associação com diferentes veículos frente a patógenos endodônticos. O teste de difusão em caldo foi utilizado. Foram preparadas pastas com pó de Ca(OH)2 e os seguintes veículos: água destilada, glicerina, PMCC (paramonoclorofenol canforado), PMCC + glicerina, e PMCC + polietilenoglicol. O tempo necessário para as pastas produzirem culturas negativas frente aos microorganismos testados foi registrado e analisado estatisticamente por meio do teste de Kruskal Wallis com nível de significância de 5%. Foram necessárias de 6 a 24 h para eliminar os microrganismos aeróbios e facultativos e de 30 s a 5 min para os anaeróbios estritos. A suscetibilidade microbiana em ordem crescente foi: Enterococcus faecalis (patógeno mais resistente), Candida albicans, Staphylococcus aureus, Porphyromonas gingivalis, Porphyromonas endodontalis e Prevotella intermedia (os últimos dois microrganismos necessitaram do mesmo tempo para serem eliminados). Concluiu-se que as pastas de hidróxido de cálcio necessitaram de maior tempo para eliminar os microrganismos facultativos do que os anaeróbios. Esses achados sugerem que a propriedade antimicrobiana está relacionada tanto à formulação da pasta quanto à suscetibilidade microbiana.
Subject: Endodontics
intracanal medication
calcium hydroxide
bacteria
Editor: Fundação Odontológica de Ribeirão Preto
Rights: aberto
Identifier DOI: 10.1590/S0103-64402005000300001
Address: http://dx.doi.org/10.1590/S0103-64402005000300001
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402005000300001
Date Issue: 1-Dec-2005
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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