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Type: Artigo
Title: Venom of the Phoneutria nigriventer spider alters the cell cycle, viability, and migration of cancer cells
Author: dos Santos, Natalia Barreto
Bonfanti, Amanda Pires
Alves da Rocha-e-Silva, Thomaz Augusto
da Silva Junior, Pedro Ismael
da Cruz-Hofling, Maria Alice
Verinaud, Liana
Raposo, Catarina
Abstract: The mechanisms of cancer involve changes in multiple biological pathways. Multitarget molecules, which are components of animal venoms, are therefore a potential strategy for treating tumors. The objective of this study was to screen the effects of Phoneutria nigriventer spider venom (PnV) on tumor cell lines. Cultured human glioma (NG97), glioblastoma (U-251) and cervix adenocarcinoma (HeLa) cells, and nontumor mouse fibroblasts (L929) were treated with low (14 mu g/ml) and high (280 mu g/ml) concentrations of PnV, and analyzed through assays for cell viability (thiazolyl blue tetrazolium blue), proliferation (carboxyfluorescein succinimidyl ester), death (annexin V/propidium iodide [Pi]), the cell cycle (Pi), and migration (wound healing and transwell assay). The venom decreased the viability of U-251 cells, primarily by inducing cell death, and reduced the viability of NG97 cells, primarily by inhibiting the cell cycle. The migration of all the tumor cell lines was delayed when treated with venom. The venom significantly affected all the tumor cell lines studied, with no cytotoxic effect on normal cells (L929), although the nonglial tumor cell (HeLa) was less sensitive to PnV. The results of the current study suggest that PnV may be composed of peptides that are highly specific for the multiple targets involved in the hallmarks of cancer. Experiments are underway to identify these molecules
Subject: Cancer - Tratamento
Técnicas in vitro
Country: Estados Unidos
Editor: John Wiley & Sons
Rights: Fechado
Identifier DOI: 10.1002/jcp.26935
Date Issue: 2019
Appears in Collections:IB - Artigos e Outros Documentos
FCF - Artigos e Outros Documentos

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