Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/332
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.subject.otherMYELODYSPLASTIC SYNDROMESen
dc.subject.otherGENE-EXPRESSIONen
dc.subject.otherANTISENSE RNAen
dc.subject.otherBONE-MARROWen
dc.subject.otherHEMATOPOIETIC MICROENVIRONMENTen
dc.subject.otherMYELOID-LEUKEMIAen
dc.subject.otherPROGENITOR CELLSen
dc.subject.otherTRANSCRIPTIONen
dc.subject.otherAPOPTOSISen
dc.subject.otherCANCERen
dc.typeArtigo de periódicopt_BR
dc.type.categoryoriginal articlept_BR
dc.type.versionpublishedVersionpt_BR
dc.titleIdentification of protein-coding and non-coding RNA expression profiles in CD34(+) and in stromal cells in refractory anemia with ringed sideroblastspt_BR
dc.contributor.authorBARATTI, Mariana O.pt_BR
dc.contributor.authorMOREIRA, Yuri B.pt_BR
dc.contributor.authorTRAINA, Fabiolapt_BR
dc.contributor.authorCOSTA, Fernando F.pt_BR
dc.contributor.authorVERJOVSKI-ALMEIDA, Sergiopt_BR
dc.contributor.authorOLALLA-SAAD, Sara T.pt_BR
unicamp.authorBARATTI, Mariana O.:Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Departamento de Medicina Internapt_BR
unicamp.authorTRAINA, Fabiola:Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Departamento de Medicina Internapt_BR
unicamp.authorCOSTA, Fernando F.:pt_BR
unicamp.author.externalMOREIRA, Yuri B.pt
unicamp.author.externalVERJOVSKI-ALMEIDA, Sergiopt
dc.subject.wosGenetics & Hereditypt_BR
dc.description.abstractBackground: Myelodysplastic syndromes (MDS) are a group of clonal hematological disorders characterized by ineffective hematopoiesis with morphological evidence of marrow cell dysplasia resulting in peripheral blood cytopenia. Microarray technology has permitted a refined high-throughput mapping of the transcriptional activity in the human genome. Non-coding RNAs (ncRNAs) transcribed from intronic regions of genes are involved in a number of processes related to post-transcriptional control of gene expression, and in the regulation of exon-skipping and intron retention. Characterization of ncRNAs in progenitor cells and stromal cells of MDS patients could be strategic for understanding gene expression regulation in this disease. Methods: In this study, gene expression profiles of CD34(+) cells of 4 patients with MDS of refractory anemia with ringed sideroblasts (RARS) subgroup and stromal cells of 3 patients with MDS-RARS were compared with healthy individuals using 44 k combined intron-exon oligoarrays, which included probes for exons of protein-coding genes, and for non-coding RNAs transcribed from intronic regions in either the sense or antisense strands. Real-time RT-PCR was performed to confirm the expression levels of selected transcripts. Results: In CD34(+) cells of MDS-RARS patients, 216 genes were significantly differentially expressed (q-value <= 0.01) in comparison to healthy individuals, of which 65 (30%) were non-coding transcripts. In stromal cells of MDS-RARS, 12 genes were significantly differentially expressed (q-value <= 0.05) in comparison to healthy individuals, of which 3 (25%) were non-coding transcripts. Conclusions: These results demonstrated, for the first time, the differential ncRNA expression profile between MDS-RARS and healthy individuals, in CD34(+) cells and stromal cells, suggesting that ncRNAs may play an important role during the development of myelodysplastic syndromes.en
dc.relation.ispartofBMC Medical Genomicspt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherBIOMED CENTRAL LTDpt_BR
dc.date.issued2010pt_BR
dc.identifier.citationBMC MEDICAL GENOMICS, v.3, 2010pt_BR
dc.language.isoengpt_BR
dc.description.volume3pt_BR
dc.rightsabertopt_BR
dc.rights.holderCopyright BIOMED CENTRAL LTDpt_BR
dc.sourceWOSpt_BR
unicamp.cruespUSPpt_BR
dc.identifier.issn1755-8794pt_BR
dc.identifier.doi10.1186/1755-8794-3-30pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1186/1755-8794-3-30pt_BR
dc.identifier.urlhttp://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000283178500001&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecordpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorremissiveCNPq_Brasilpt_BR
dc.description.sponsorremissiveFAPESP_Brasilpt_BR
dc.date.available2013-07-26T17:57:36Z
dc.date.available2016-07-01T15:42:48Z-
dc.date.accessioned2013-07-26T17:57:36Z
dc.date.accessioned2016-07-01T15:42:48Z-
dc.description.provenanceMade available in DSpace on 2013-07-26T17:57:36Z (GMT). No. of bitstreams: 2 art_BARATTI_Identification_of_protein-coding_and_non-coding_RNA_expression_2010.pdf.txt: 55881 bytes, checksum: c54ea741add53cb4d4a5276f4cae52ea (MD5) art_BARATTI_Identification_of_protein-coding_and_non-coding_RNA_expression_2010.pdf: 3932206 bytes, checksum: fd2ff79a8215f7603309535b9cf116c8 (MD5) Previous issue date: 2010en
dc.description.provenanceMade available in DSpace on 2016-07-01T15:42:48Z (GMT). No. of bitstreams: 2 art_BARATTI_Identification_of_protein-coding_and_non-coding_RNA_expression_2010.pdf.txt: 55881 bytes, checksum: c54ea741add53cb4d4a5276f4cae52ea (MD5) art_BARATTI_Identification_of_protein-coding_and_non-coding_RNA_expression_2010.pdf: 3932206 bytes, checksum: fd2ff79a8215f7603309535b9cf116c8 (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/332
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/332-
dc.contributor.departmentMedicina Interna
dc.contributor.unidadeFCMpt
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