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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authoremailalanroger@fop.unicamp.brpt_BR
dc.typeArtigopt_BR
dc.titleTissue Microarray Use For Immunohistochemical Study Of Ameloblastomaen
dc.contributor.authorNeves-Silvapt_BR
dc.contributor.authorRodrigo; Fonsecapt_BR
dc.contributor.authorFelipe Paiva; de Jesuspt_BR
dc.contributor.authorAdriana Souza; Rebelo Pontespt_BR
dc.contributor.authorHelder Antonio; Rochapt_BR
dc.contributor.authorAndre Caroli; Brandaopt_BR
dc.contributor.authorThais Bianca; Vargaspt_BR
dc.contributor.authorPablo Agustin; Lopespt_BR
dc.contributor.authorMarcio Ajudarte; de Almeidapt_BR
dc.contributor.authorOslei Paes; Santos-Silvapt_BR
dc.contributor.authorAlan Rogerpt_BR
unicamp.authorSantos-Silva, Alan Roger] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Oral Diag, Semiol & Pathol, Sao Paulo, Brazilpt_BR
unicamp.author.external[Neves-Silva, Rodrigopt_BR
unicamp.author.externalFonseca, Felipe Paivapt_BR
unicamp.author.externalBrandao, Thais Biancapt_BR
unicamp.author.externalVargas, Pablo Agustinpt_BR
unicamp.author.externalLopes, Marcio Ajudartept_BR
unicamp.author.externalde Almeida, Oslei Paespt_BR
unicamp.author.external[de Jesus, Adriana Souzapt_BR
unicamp.author.externalRebelo Pontes, Helder Antonio] Fed Univ Para, Serv Oral Pathol, Joao de Barros Barreto Univ Hosp, Belem, Para, Brazilpt_BR
unicamp.author.external[Rocha, Andre Caroli] Univ Sao Paulo, Med Sch, Clin Hosp, Sao Paulo, Brazilpt_BR
unicamp.author.external[Vargas, Pablo Agustin] Univ Pretoria, Dept Oral Pathol & Oral Biol, Sch Dent, Fac Hlth Sci, Pretoria, South Africapt_BR
dc.subjectAmeloblastomaen
dc.subjectBcl-2en
dc.subjectCytokeratinen
dc.subjectKi67en
dc.subjectTissue Microarrayen
dc.description.abstractAmeloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. MethodsForty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). ResultsKappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). ConclusionUsing a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.en
dc.relation.ispartofJournal of Oral Pathology & Medicinept_BR
dc.publisherWiley-Blackwellpt_BR
dc.publisherHobokenpt_BR
dc.date.issued2016pt_BR
dc.date.monthofcirculationoutpt_BR
dc.identifier.citationJournal Of Oral Pathology & Medicine. Wiley-blackwell, v. 45, p. 704 - 711, 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume45pt_BR
dc.description.issuenumber9pt_BR
dc.description.firstpage704pt_BR
dc.description.lastpage711pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0904-2512pt_BR
dc.identifier.eissn1600-0714pt_BR
dc.identifier.wosidWOS:000385713700011pt_BR
dc.identifier.doi10.1111/jop.12428pt_BR
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/jop.12428/abstractpt_BR
dc.date.available2017-11-13T13:58:05Z-
dc.date.accessioned2017-11-13T13:58:05Z-
dc.description.provenanceMade available in DSpace on 2017-11-13T13:58:05Z (GMT). No. of bitstreams: 1 000385713700011.pdf: 390630 bytes, checksum: ac0623dac595d2c29369854309e08127 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/330190-
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