Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/330190
Type: Artigo
Title: Tissue Microarray Use For Immunohistochemical Study Of Ameloblastoma
Author: Neves-Silva
Rodrigo; Fonseca
Felipe Paiva; de Jesus
Adriana Souza; Rebelo Pontes
Helder Antonio; Rocha
Andre Caroli; Brandao
Thais Bianca; Vargas
Pablo Agustin; Lopes
Marcio Ajudarte; de Almeida
Oslei Paes; Santos-Silva
Alan Roger
Abstract: Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. MethodsForty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). ResultsKappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). ConclusionUsing a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.
Subject: Ameloblastoma
Bcl-2
Cytokeratin
Ki67
Tissue Microarray
Editor: Wiley-Blackwell
Hoboken
Rights: fechado
Identifier DOI: 10.1111/jop.12428
Address: http://onlinelibrary.wiley.com/doi/10.1111/jop.12428/abstract
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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