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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authoremailkathleen-sluka@uiowa.edupt_BR
dc.typeArtigopt_BR
dc.titleAsic3 Is Required For Development Of Fatigue-induced Hyperalgesiaen
dc.contributor.authorGregorypt_BR
dc.contributor.authorNicholas S.; Britopt_BR
dc.contributor.authorRenan G.; Oliveira Fusaropt_BR
dc.contributor.authorMaria Claudia G.; Slukapt_BR
dc.contributor.authorKathleen A.pt_BR
unicamp.author[Oliveira Fusaro, Maria Claudia G.] State Univ Campinas UNICAMP, Fac Ciencias Aplicadas, Campinas, SP, Brazilpt_BR
unicamp.author.external[Gregory, Nicholas S.pt_BR
unicamp.author.externalSluka, Kathleen A.] Univ Iowa, Neurosci Grad Program, Pain Res Program, Dept Phys Therapy & Rehabil Sci, 1-248 MEB, Iowa City, IA 52242 USApt_BR
unicamp.author.external[Brito, Renan G.] Univ Fed Sergipe, Dept Physiol, Aracaju, Brazilpt_BR
dc.subjectPainen
dc.subjectExerciseen
dc.subjectProtonen
dc.subjectAciden
dc.subjectAsicen
dc.subjectHyperalgesiaen
dc.subjectMacrophageen
dc.description.abstractAn acute bout of exercise can exacerbate pain, hindering participation in regular exercise and daily activities. The mechanisms underlying pain in response to acute exercise are poorly understood. We hypothesized that proton accumulation during muscle fatigue activates acid-sensing ion channel 3 (ASIC3) on muscle nociceptors to produce hyperalgesia. We investigated the role of ASIC3 using genetic and pharmacological approaches in a model of fatigue-enhanced hyperalgesia. This model uses two injections of pH 5.0 saline into muscle in combination with an electrically induced fatigue of the same muscle just prior to the second injection of acid to induce mechanical hyperalgesia. We show a significant decrease in muscle force and decrease in muscle pH after 6 min of electrical stimulation. Genetic deletion of ASIC3 using knockout mice and pharmacological blockade of ASIC3 with APETx2 in muscle prevents the fatigue-enhanced hyperalgesia. However, ASIC3(-/-) mice and APETx2 have no effect on the fatigue response. Genetic deletion of ASIC3 in primary afferents innervating muscle using an HSV-1 expressing microRNA (miRNA) to ASIC3 surprisingly had no effect on the development of the hyperalgesia. Muscle fatigue increased the number of macrophages in muscle, and removal of macrophages from muscle with clodronate liposomes prevented the development of fatigue-enhanced hyperalgesia. Thus, these data suggest that fatigue reduces pH in muscle that subsequently activates ASIC3 on macrophages to enhance hyperalgesia to muscle insult.en
dc.relation.ispartofMolecular Neurobiologypt_BR
dc.publisherHumana Press Incpt_BR
dc.publisherTotowapt_BR
dc.date.issued2016pt_BR
dc.date.monthofcirculationmarpt_BR
dc.identifier.citationMolecular Neurobiology. Humana Press Inc, v. 53, p. 1020 - 1030, 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume53pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage1020pt_BR
dc.description.lastpage1030pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0893-7648pt_BR
dc.identifier.eissn1559-1182pt_BR
dc.identifier.wosidWOS:000370187100018pt_BR
dc.identifier.doi10.1007/s12035-014-9055-4pt_BR
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs12035-014-9055-4pt_BR
dc.description.sponsorshipNational Institute of Health [AR061371, AR053509]pt_BR
dc.date.available2017-11-13T13:57:57Z-
dc.date.accessioned2017-11-13T13:57:57Z-
dc.description.provenanceMade available in DSpace on 2017-11-13T13:57:57Z (GMT). No. of bitstreams: 1 000370187100018.pdf: 804989 bytes, checksum: f57f95a2f691f1a60a471c75ec745a93 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/330153-
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