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Type: Artigo
Title: Fish And Agnor Mapping Of The 45s And 5s Rrna Genes In Wild And Cultivated Species Of Capsicum (solananceae)
Author: Scaldaferro
Marisel A.; Victoria Romero da Cruz
M.; Cecchini
Nicolas M.; Moscone
Eduardo A.
Abstract: Chromosome number and position of rDNA were studied in 12 wild and cultivated species of the genus Capsicum with chromosome numbers x = 12 and x = 13 (22 samples). For the first time in these species, the 5S and 45S rRNA loci were localized and physically mapped using two-color fluorescence in situ hybridization and AgNOR banding. We focused on the comparison of the results obtained with both methods with the aim of accurately revealing the real functional rRNA genes. The analyzes were based on a previous work that reported that the 18S-5.8S-25S loci mostly coincide with GC-rich heterochromatic regions and likely have given rise to satellite DNAs, which are not active genes. These data show the variability of rDNA within karyotypes of the genus Capsicum, providing anchor points for (comparative) genetic maps. In addition, the obtained information might be useful for studies on evolution of repetitive DNA.
Subject: Capsicum Chromosomes
Fluorescence In Situ Hybridization
Agnor Banding
5s Rrna Genes
45s Rrna Genes
Physical Gene Mapping
Editor: Canadian Science Publishing, Nrc Research Press
Citation: Genome. Canadian Science Publishing, Nrc Research Press, v. 59, p. 95 - 113, 2016.
Rights: fechado
Identifier DOI: 10.1139/gen-2015-0099
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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