Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/330065
Type: Artigo
Title: Implication Of Rho-kinase And Soluble Guanylyl Cyclase Enzymes In Prostate Smooth Muscle Dysfunction In Middle-aged Rats
Implication of Rho-kinase and soluble guanylyl cyclase enzymes in prostate smooth muscle dysfunction in middle-aged rats
Author: Calmasini, Fabiano B.
Silva, Fabio H.
Alexandre, Eduardo C.
Rodrigues, Renata L.
Barbosa, Ana Paula L.
Ferrucci, Danilo L.
Carvalho, Hernandes F.
Anhe, Gabriel F.
Pupo, Andre S.
Antunes, Edson
Abstract: Aging is highly associated with benign prostate hyperplasia (BPH). We investigated here the alterations of the contractile and relaxant machinery in prostates of middle-aged rats, focusing on the Rho-kinase, nitric oxide (NO)-soluble guanylyl cyclase (sGC), 1- and -adrenoceptor pathways.Methods: Male Wistar young (3.5-month old) and middle-aged rats (10-month old) were used. Quantitative image analysis of prostates and functional assays evaluating the prostate contractions and relaxations were employed. Measurement of [H-3]-noradrenaline efflux, western blotting for 1 and 1 sGC subunits, and cyclic nucleotide levels were carried out.Results: Prostates of middle-aged rats showed significant increases in lumen and smooth muscle cells, but no alterations in the relative prostate weight were observed. In vivo, noradrenaline (10(-7)-10(-4)g/kg) produced greater prostatic contractions in middle-aged compared with control rats. Likewise, the in vitro contractions to phenylephrine (1nM-100M) and ,-methylene ATP (1-10M) were greater in middle-aged rats. Electrical-field stimulation (EFS, 1-32Hz) promoted higher [H-3]-noradrenaline efflux and prostate contractions in middle-aged rats. Reduced expressions of 1 and 1 sGC subunits and diminished NO-mediated prostate relaxations in middle-age were observed. Isoproterenol-induced relaxations and cAMP levels were reduced in prostates of middle-aged rats. The Rho-kinase inhibitor fasudil (50mg/kg, 2 weeks) normalized the prostate hypercontractility in middle-age rats.Conclusions: Prostate hypercontractility in middle-aging is associated with increased release of noradrenaline and Rho-kinase pathway, as well as with impairments of NO-sGC and -adrenoceptor pathways. Middle-aged rats are suitable to explore the enhanced prostatic tone in the absence of prostate overgrowth. Neurourol. Urodynam. (C) 2016 Wiley Periodicals, Inc.
Aging is highly associated with benign prostate hyperplasia (BPH). We investigated here the alterations of the contractile and relaxant machinery in prostates of middle-aged rats, focusing on the Rho-kinase, nitric oxide (NO)-soluble guanylyl cyclase (sGC), 1- and -adrenoceptor pathways.Methods: Male Wistar young (3.5-month old) and middle-aged rats (10-month old) were used. Quantitative image analysis of prostates and functional assays evaluating the prostate contractions and relaxations were employed. Measurement of [H-3]-noradrenaline efflux, western blotting for 1 and 1 sGC subunits, and cyclic nucleotide levels were carried out.Results: Prostates of middle-aged rats showed significant increases in lumen and smooth muscle cells, but no alterations in the relative prostate weight were observed. In vivo, noradrenaline (10(-7)-10(-4)g/kg) produced greater prostatic contractions in middle-aged compared with control rats. Likewise, the in vitro contractions to phenylephrine (1nM-100M) and ,-methylene ATP (1-10M) were greater in middle-aged rats. Electrical-field stimulation (EFS, 1-32Hz) promoted higher [H-3]-noradrenaline efflux and prostate contractions in middle-aged rats. Reduced expressions of 1 and 1 sGC subunits and diminished NO-mediated prostate relaxations in middle-age were observed. Isoproterenol-induced relaxations and cAMP levels were reduced in prostates of middle-aged rats. The Rho-kinase inhibitor fasudil (50mg/kg, 2 weeks) normalized the prostate hypercontractility in middle-age rats.Conclusions: Prostate hypercontractility in middle-aging is associated with increased release of noradrenaline and Rho-kinase pathway, as well as with impairments of NO-sGC and -adrenoceptor pathways. Middle-aged rats are suitable to explore the enhanced prostatic tone in the absence of prostate overgrowth
Subject: Aging
Beta-adrenoceptors
Cyclic Amp
Protein Kinase C
Stereology
Envelhecimento
AMP cíclico
Proteína Quinase C
Country: Estados Unidos
Editor: Wiley-Liss
Citation: Neurourology And Urodynamics. Wiley-blackwell, v. 36, p. 589 - 596, 2017.
Rights: aberto
Identifier DOI: 10.1002/nau.22990
Address: http://onlinelibrary.wiley.com/doi/10.1002/nau.22990/abstract
Date Issue: 2017
Appears in Collections:IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos

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