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Type: Artigo
Title: Development Of A Water-in-oil-in-water Multiple Emulsion System Integrating Biomimetic Aqueous-core Lipid Nanodroplets For Protein Entity Stabilization. Part Ii: Process And Product Characterization
Author: Glasser
Cassia A.; Vila
Marta M. D. C.; Pereira
Julio C.; Chaud
Marco V.; Oliveira Junior
Jose M.; Tubino
Matthieu; Balcao
Victor M.
Abstract: The aqueous-core enclosed in lipid nanoballoons integrating multiple emulsions of the type water-in-oil-in-water mimic, at least in theory, the environment within viable cells, thus being suitable for housing hydrophilic protein entities such as bioactive proteins, peptides and bacteriophage particles. This study reports a complete physicochemical characterization of optimized biomimetic aqueous-core lipid nanoballoons housing hydrophilic (BSA) protein entities, evolved from a statistical 2(3)x3(1) factorial design study (three variables at two levels and one variable at three levels) that was the subject of the first paper of a series of three, aiming at complete stabilization of the three-dimensional structure of protein entities attempted via housing the said molecular entities within biomimetic aqueous-core lipid nanoballoons integrating a multiple (W/O/W) emulsion. The statistical factorial design followed led to the production of an optimum W/O/W multiple emulsion possessing quite homogeneous particles with an average hydrodynamic size of (186.2 +/- 2.6) nm and average Zeta potential of (-36.5 +/- 0.9) mV, and exhibiting a polydispersity index of 0.206 +/- 0.014. Additionally, the results obtained for the diffusion coefficient of the lipid nanoballoons integrating the optimized W/O/W multiple emulsion were comparable and of the same order of magnitude (10(-12) m(2)s(-1)) as those published by other authors since, typically, diffusion coefficients for molecules range from 10(-10) to 10(-7) m(2)s(-1), but diffusion coefficients for nanoparticles are typically of the order of magnitude of 10(-12) m(2)s(-1).
Subject: Aqueous-core Lipid Nanoballoons
Diffusion Coefficient
Infrared Spectrophotometry With Fourier Transform
Protein Entities
Stokes-einstein Equation
Structural And Functional Stabilization
Water-in-oil-in-water Multiple Emulsions
X-ray Diffraction Analysis
Editor: Taylor & Francis Ltd
Rights: fechado
Identifier DOI: 10.1080/03639045.2016.1188109
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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