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Type: Artigo
Title: Hypothalamic Stearoyl-coa Desaturase-2 (scd2) Controls Whole-body Energy Expenditure
Author: de Moura
R. F.; Nascimento
L. F.; Ignacio-Souza
L. M.; Morari
J.; Razolli
D. S.; Solon
C.; de Souza
G. F. P.; Festuccia
W. T.; Velloso
L. A.
Abstract: Stearoyl-CoA desaturase-2 (SCD2) is the main delta 9 desaturase expressed in the central nervous system. Because of its potential involvement in controlling whole-body adiposity, we evaluated the expression and function of SCD2 in the hypothalami of mice. SUBJECTS/METHODS: Male mice of different strains were used in real-time PCR, immunoblot and metabolic experiments. In addition, antisense oligonucleotides and lentiviral vectors were used to reduce and increase the expression of SCD2 in the hypothalamus. RESULTS: The level of SCD2 in the hypothalamus is similar to other regions of the central nervous system and is similar to 10-fold higher than in any other region of the body. In the arcuate nucleus, SCD2 is expressed in proopiomelanocortin and neuropeptide-Y neurons. Upon high fat feeding, the level of hypothalamic SCD2 increases. Inhibition of hypothalamic SCD2 as accomplished by two distinct approaches, an antisense oligonucleotide or a short-hairpin RNA delivered by a lentivirus, resulted in reduced body mass gain mostly due to increased energy expenditure and increased spontaneous activity. Increasing hypothalamic SCD2 by a lentivirus approach resulted in no change in body mass and food intake. CONCLUSIONS: Thus, SCD2 is highly expressed in the hypothalami of rodents and its knockdown reduces body mass due to increased whole-body energy expenditure.
Subject: Insulin-resistance;gene-expression;mediterranean Diet;obesity;fat;activation;inflammation;prevention;coenzyme;rodents
Editor: Nature Publishing Group
Rights: fechado
Identifier DOI: 10.1038/ijo.2015.188
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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