Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/329722
Type: Artigo
Title: Development And Characterization Of A New Adsorbent For Biomolecule Separation: Intercalation And Adsorption Of Clavulanic Acid In Layered Double Hydroxides
Author: Soares Forte
Marcus Bruno; Taviot-Gueho
Christine; Leroux
Fabrice; Rodrigues
Maria Isabel; Maugeri Filho
Francisco
Abstract: A new layered double hydroxide (LDH) material has been developed through a pioneering approach, with the goal of being an alternative adsorbent for the separation of biomolecules, such as clavulanic acid (CA). RESULTS: Zn2Cr-CA hybrid LDH assemblies were prepared through a coprecipitation method to evaluate the affinity between the CA molecule and the interlayer sites. The resulting inorganic-organic 'sandwich' structure was characterized by a combination of techniques showing an expansion of the layered structure from 0.78 nm up to values of 2.30 nm upon CA intercalation. Isotherm studies were carried out to evaluate the adsorption capacity of LDH towards CA. The Freundlich adsorption model was found to fit the experimental data well, indicating a relatively large adsorption capacity for the adsorbent Zn2Cr-NO3, as high as 73.63, 128.76, and 229.62 mg(1-nF)L(nF)g(-1) at 20, 30, and 35 degrees C, respectively. CONCLUSION: Zn2Cr-NO3 adsorption capacity was not influenced by the presence of amino acids, thus underlining that it is a LDH composition which may be a suitable alternative for the separation of CA. LDH micro-particles were additionally encapsulated with calcium alginate gel (LDHME) and characterized for particle average diameter (d(p) = 171 mu m), bulk density (rho(bulk) = 0.63 g cm(-3)), particle density (rho(p) = 2.2 g cm(-3)), particle porosity (epsilon(p) = 0.71) and bed porosity (epsilon(B) = 0.40). The application of LDHME in the separation of CA from complex mixtures, containing tyrosine and proline, was considered satisfactory based on purification factors of about 2.32 and degrees of purity higher than 99%. (C) 2015 Society of Chemical Industry
Subject: Clavulanic Acid
Layered Double Hydroxides
Intercalation
Adsorption
Equilibrium Isotherms
Separation
Fixed Bed Chromatography
Purification
Editor: Wiley-Blackwell
Hoboken
Rights: fechado
Identifier DOI: 10.1002/jctb.4759
Address: http://onlinelibrary.wiley.com/doi/10.1002/jctb.4759/full
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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