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|Title:||Laser Ablation And Inductively Coupled Plasma Mass Spectrometry Focusing On Bioimaging From Elemental Distribution Using Matlab Software: A Practical Guide|
Gustavo de S.; Capelo-Martinez
Jose L.; Fdez-Riverola
Marco A. Z.
|Abstract:||The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.|
Solution-based Calibration;biological Samples;trace-elements;tissue;strategies;fixation;section;metals;zinc
|Editor:||Royal Soc Chemistry|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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