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Type: Artigo
Title: Integrated Analysis Of Recombinant Spv-1 L1 Protein For The Production Of A Bovine Papillomavirus Vlp Vaccine
Author: Modolo
Diego Grando; Araldi
Rodrigo Pinheiro; Mazzuchelli-de-Souza
Jacqueline; Pereira
Alexandre; Pimenta
Daniel Carvalho; Zanphorlin
Leticia Maria; Becak
Willy; Menossi
Marcelo; Stocco
Rita de Cassia; de Carvalho
Rodrigo Franco
Abstract: Bovine papillomatosis is an infectious disease that is caused by bovine papillomavirus (BPV), which results in important economic losses. However, no BPV vaccines or effective treatment methods are commercially available to date. Moreover, the absence of papillomavirus replication in vitro makes the use of recombinant protein a promising candidate for vaccine formulations. Hence, we developed an integrated study on the L1 capsid protein of BPV-1, obtained from a bacterial expression system, regarding its purification, biosafety, thermostability and immunogenicity. The results indicated an absence of genotoxicity of the purified recombinant L1 protein, beta-sheet prevalence of secondary structure folding, protein stability under high temperatures as well as the presence of capsomeres and VLPs. In addition, preliminary experimental vaccination of calves showed the production of specific antibodies against BPV-1 L1. (C) 2017 Elsevier Ltd. All rights reserved.
Subject: Bovine Papillomavirus
L1 Protein
Editor: Elsevier Sci LTD
Rights: fechado
Identifier DOI: 10.1016/j.vaccine.2017.02.007
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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