Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/329581
Type: Artigo
Title: The Functional Properties Of A Xyloglucanase (gh12) Of Aspergillus Terreus Expressed In Aspergillus Nidulans May Increase Performance Of Biomass Degradation
The functional properties of a xyloglucanase (gh12) of Aspergillus terreus expressed in Aspergillus nidulans may increase performance of biomass degradation
Author: Vitcosque, Gabriela Leal
Ribeiro, Liliane Fraga Costa
Lucas, Rosymar Coutinho de
Silva, Tony Marcio da
Ribeiro, Lucas Ferreira
Damasio, André Ricardo de Lima
Farinas, Cristiane Sanchez
Gonçalves, Aline Zorzetto Lopes
Segato, Fernan
Abstract: Filamentous fungi are attractive hosts for heterologous protein expression due to their capacity to secrete large amounts of enzymes into the extracellular medium. Xyloglucanases, which specifically hydrolyze xyloglucan, have been recently applied in lignocellulosic biomass degradation and conversion in many other industrial processes. In this context, this work aimed to clone, express, and determine the functional properties of a recombinant xyloglucanase (AtXEG12) from Aspergillus terreus, and also its solid-state (SSF) and submerged (SmF) fermentation in bioreactors. The purified AtXEG12 showed optimum pH and temperature of 5.5 and 65 A degrees C, respectively, demonstrating to be 90 % stable after 24 h of incubation at 50 A degrees C. AtXEG12 activity increased in the presence of 2-mercaptoethanol (65 %) and Zn+2 (45 %), while Cu+2 and Ag+ ions drastically decreased its activity. A substrate assay showed, for the first time for this enzyme's family, xylanase activity. The enzyme exhibited high specificity for tamarind xyloglucan (K (M) 1.2 mg mL(-1)) and V (max) of 17.4 mu mol min(-1) mg(-1) of protein. The capillary zone electrophoresis analysis revealed that AtXEG12 is an endo-xyloglucanase. The heterologous xyloglucanase secretion was greater than the production by wild-type A. terreus cultivated in SmF. On the other hand, AtXEG12 activity reached by SSF was sevenfold higher than values achieved by SmF, showing that the expression of recombinant enzymes can be significantly improved by cultivation under SSF.
Filamentous fungi are attractive hosts for heterologous protein expression due to their capacity to secrete large amounts of enzymes into the extracellular medium. Xyloglucanases, which specifically hydrolyze xyloglucan, have been recently applied in lign
Subject: Aspergillus Terreus
Xyloglucanases
Ssf
Smf
Recombinant Enzymes
Aspergillus
Fungos filamentosos
Proteínas recombinantes
Expressão heteróloga
Fermentação em estado sólido
Fermentação submersa
Country: Alemanha
Editor: Springer
Citation: Applied Microbiology And Biotechnology. Springer, v. 100, p. 9133 - 9144, 2016.
Rights: fechado
fechado
Identifier DOI: 10.1007/s00253-016-7589-2
Address: https://link.springer.com/article/10.1007/s00253-016-7589-2
Date Issue: 2016
Appears in Collections:IB - Artigos e Outros Documentos

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