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|Title:||A Minimally Invasive Approach To Spleen Histopathology In Dogs: A New Method For Follow-up Studies Of Spleen Changes In The Course Of Leishmania Infantum Infection|
Silvana Ornelas; Fontes
Jonathan L. M.; Laranjeira
Daniela F.; Vassallo
Stella Maria; dos-Santos
Washington L. C.
|Abstract:||Severe forms of zoonotic visceral leishmaniosis (ZVL) are associated with disruption of the spleen structure. However, the study of spleen histology requires splenectomy or necropsy. In this work, we present a minimally invasive cell-block technique for studying spleen tissue histology in dogs with ZVL. We examined 13 dogs with and seven dogs without Leishmania infantum infection. The dogs with Leishmania infection had a lower frequency of lymphoid follicles (2/13, Fisher's test, P<0.02) and a higher density of plasma cells (score 3, Fisher's test, P<0.02) than uninfected dogs (5/7 exhibiting lymphoid follicles and a plasma cell score of 1). The dogs with Leishmania infection also presented with granulomas (8/13) and infected macrophages (5/13). These differences in the histological presentations of spleen tissue from infected and uninfected dogs corresponded to changes observed in conventional histology. Hence, the cell-block technique described here may be used in the follow-up care and study of dogs with ZVL and other diseases in both clinical practice and research. (C) 2016 Elsevier Ltd. All rights reserved.|
Canine Visceral Leishmaniasis
Minimally Invasive Histopathology
|Editor:||Elsevier Sci LTD|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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