Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/329209
Type: Artigo
Title: Development And Evaluation Of A Novel Mucoadhesive Film Containing Acmella Oleracea Extract For Oral Mucosa Topical Anesthesia
Development and evaluation of a novel mucoadhesive film containing Acmella oleracea extract for oral mucosa topical anesthesia
Author: de Freitas-Blanco, Veronica Santana
Franz-Montan, Michelle
Groppo, Francisco Carlos
de Carvalho, Joao Ernesto
Figueira, Glyn Mara
Serpe, Luciano
Oliveira Sousa, Ilza Maria
Guilherme Damasio, Viviane Aparecida
Yamane, Lais Thiemi
de Paula, Eneida
Ferreira Rodrigues, Rodney Alexandre
Abstract: To develop an anesthetic mucoadhesive film containing Acmella oleracea (jambu) extract for topical use on oral mucosa. Methods Ethanolic extracts from aerial parts of jambu were prepared by maceration. Pigment removal was obtained by adsorption with activated carbon. Three mucoadhesive films were developed using a film casting method: 10 or 20% of crude jambu extract (10% JB and 20% JB), and 10% of crude jambu extract treated with activated carbon (10% JBC). The mucoadhesive films were characterized regarding their uniformity, thickness, pH, and spilanthol content, and their stability was evaluated during 120 days. Gas chromatography was used to quantify the amount of spilanthol. In vitro tests determined the permeation of spilanthol across pig esophageal epithelium mucosa in Franz diffusion cells. Topical anesthetic efficacy was assessed in vivo using a tail flick test in mice. Results The three mucoadhesive films showed physical stability and visual appearances suitable for use on oral mucosa. The permeation study revealed that the spilanthol from 10% JBC presented higher flux and permeability coefficient values, compared to 10% or 20% JB (p < 0.001). Moreover, 10% JBC showed better topical anesthetic efficacy than the other films (p < 0.01). Conclusion Mucoadhesive film containing crude extract of jambu treated with activated carbon is a potential alternative for oral, topical use, encouraging future clinical studies.
To develop an anesthetic mucoadhesive film containing Acmella oleracea (jambu) extract for topical use on oral mucosa. Methods Ethanolic extracts from aerial parts of jambu were prepared by maceration. Pigment removal was obtained by adsorption with activated carbon. Three mucoadhesive films were developed using a film casting method: 10 or 20% of crude jambu extract (10% JB and 20% JB), and 10% of crude jambu extract treated with activated carbon (10% JBC). The mucoadhesive films were characterized regarding their uniformity, thickness, pH, and spilanthol content, and their stability was evaluated during 120 days. Gas chromatography was used to quantify the amount of spilanthol. In vitro tests determined the permeation of spilanthol across pig esophageal epithelium mucosa in Franz diffusion cells. Topical anesthetic efficacy was assessed in vivo using a tail flick test in mice. Results The three mucoadhesive films showed physical stability and visual appearances suitable for use on oral mucosa. The permeation study revealed that the spilanthol from 10% JBC presented higher flux and permeability coefficient values, compared to 10% or 20% JB (p < 0.001). Moreover, 10% JBC showed better topical anesthetic efficacy than the other films (p < 0.01). Conclusion Mucoadhesive film containing crude extract of jambu treated with activated carbon is a potential alternative for oral, topical use, encouraging future clinical studies
Subject: Mucosa bucal
Anestésicos locais
Country: Estados Unidos
Editor: Public Library of Science
Citation: Plos One. Public Library Science, v. 11, p. , 2016.
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0162850
Address: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0162850
Date Issue: 2016
Appears in Collections:FOP - Artigos e Outros Documentos

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