Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/329080
Type: | Artigo |
Title: | Metabolomics By Nmr Spectroscopy In Plant Disease Diagnostic: Huanglongbing As A Case Study Metabolomics by NMR spectroscopy in plant disease diagnostic: huanglongbing as a case study |
Author: | Pontes, João Guilherme M. Ohashi, William Y. Brasil, Antonio J. M. Filgueras, Dr. Paulo R. Espíndola, Dr. Ana Paula D. M. Silva, Jaqueline S. Poppi, Prof. Ronei J. Coletta-Filho, Dr. Helvécio D. Tasic, Prof. Ljubica Tasic |
Abstract: | Candidatus Liberibacter spp. is the pathogen associated with Huanglongbing (HLB), a disease with an economic impact in the order of billions of dollars to the worldwide citrus industry. A key point to reduce HLB economic losses lies on early detection on asymptomatic stages of the infection by new detection methods as it is still not possible to cultivate Candidatus Liberibacter spp. in vitro, and the polymerase chain reaction (PCR) method used nowadays is not manageable in large scale. In this study, we search for metabolic biomarkers from Citrus sinensis leaves in different disease stages using a combined approach of NMR spectroscopy and chemometrics. Chemometric clustering was observed, providing excellent tools for class discrimination, with high accuracy, therefore enabling metabolic profile differentiation on disease early stages. Around 20 different key biomarkers, metabolites responsible for the clustering of each group, were identified using 2D NMR experimental data. Candidatus Liberibacter spp. is the pathogen associated with Huanglongbing (HLB), a disease with an economic impact in the order of billions of dollars to the worldwide citrus industry. A key point to reduce HLB economic losses lies on early detection on |
Subject: | Metabolomics Nmr Spectroscopy Biomarkers Huanglongbing Metabolism Affecting Agents Metabolômica Espectroscopia de ressonância magnética nuclear Huanglongbing Metabolismo |
Country: | Alemanha |
Editor: | Wiley |
Citation: | Chemistryselect. Wiley-v C H Verlag Gmbh, v. 1, p. 1176 - 1178, 2016. |
Rights: | fechado |
Identifier DOI: | 10.1002/slct.201600064 |
Address: | https://onlinelibrary.wiley.com/doi/full/10.1002/slct.201600064 |
Date Issue: | 2016 |
Appears in Collections: | IQ - Artigos e Outros Documentos |
Files in This Item:
File | Size | Format | |
---|---|---|---|
000395401600011.pdf | 661.8 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.