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Type: Artigo
Title: Changes in salivary microbiota increase volatile sulfur compounds production in healthy male subjects with academic-related chronic stress
Author: Nani, Bruno Dias
de Lima, Patricia Oliveira
Marcondes, Fernanda Klein
Groppo, Francisco Carlos
Rolim, Gustavo Sattolo
Alves de Moraes, Antonio Bento
Cogo-Mueller, Karina
Franz-Montan, Michelle
Abstract: To investigate the associations among salivary bacteria, oral emanations of volatile sulfur compounds, and academic-related chronic stress in healthy male subjects. Materials and methods Seventy-eight healthy male undergraduate dental students were classified as stressed or not by evaluation of burnout, a syndrome attributed to academic-related chronic stress. This evaluation was carried out using the Maslach Burnout Inventory-Student Survey questionnaire. Oral emanations of hydrogen sulfide, methyl mercaptan, and dimethyl sulfide were measured using an Oral Chroma(TM) portable gas chromatograph. The amounts in saliva of total bacteria and seven bacteria associated with halitosis were quantified by qPCR. The in vitro production of H2S by S. moorei and/or F. nucleatum was also measured with the Oral Chroma(TM) instrument. Results The stressed students group showed increased oral emanations of hydrogen sulfide and dimethyl sulfide, together with higher salivary Solobacterium moorei levels (p < 0.05, Mann Whitney test). There were moderate positive correlations between the following pairs of variables: Fusobacterium nucleatum and S. moorei; F. nucleatum and hydrogen sulfide; Tannerella forsythia and F. nucleatum; T. forsythia and S. moorei. These correlations only occurred for the stressed group (p < 0.05, Spearman correlation). The in vitro experiment demonstrated that S. moorei increased H2S production by F. nucleatum (p < 0.05, ANOVA and Tukey's test). Conclusion The increased amount of S. moorei in saliva, and its coexistence with F. nucleatum and T. forsythia, seemed to be responsible for increased oral hydrogen sulfide in the healthy male stressed subjects
Subject: Saliva
Country: Estados Unidos
Editor: Public Library of Science
Citation: Plos One. Public Library Science, v. 12, p. , 2017.
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0173686
Date Issue: 2017
Appears in Collections:FOP - Artigos e Outros Documentos

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