Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328873
Type: Artigo
Title: Harmine Stimulates Proliferation Of Human Neural Progenitors
Author: Dakic
Vanja; Macier
Renata de Moraes; Drummond
Hannah; Nascimento
Juliana M.; Trindade
Pablo; Rehen
Stevens K.
Abstract: Harmine is the beta-carboline alkaloid with the highest concentration in the psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants reverse the symptoms of depression by stimulating neuronal proliferation. It has been shown that Ayahuasca presents antidepressant effects in patients with depressive disorder. In the present study, we investigated the effects of harmine in cell cultures containing human neural progenitor cells (hNPCs, 97% nestin-positive) derived from pluripotent stem cells. After 4 days of treatment, the pool of proliferating hNPCs increased by 71.5%. Harmine has been reported as a potent inhibitor of the dual specificity tyrosine-phosphorylation regulated kinase (DYRK1A), which regulates cell proliferation and brain development. We tested the effect of analogs of harmine, an inhibitor of DYRK1A (INDY), and an irreversible selective inhibitor of monoamine oxidase (MAO) but not DYRK1A (pargyline). INDY but not pargyline induced proliferation of hNPCs similarly to harmine, suggesting that inhibition of DYRK1A is a possible mechanism to explain harmine effects upon the proliferation of hNPCs. Our findings show that harmine enhances proliferation of hNPCs and suggest that inhibition of DYRK1A may explain its effects upon proliferation in vitro and antidepressant effects in vivo.
Subject: Proliferation
Antidepressant
Dyrk1a
Hnpc
Ayahuasca
Editor: PEERJ Inc
London
Rights: aberto
Identifier DOI: 10.7717/peerj.2727
Address: https://peerj.com/articles/2727/
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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