Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328822
Type: Artigo
Title: Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries
Author: Santos, M. O.
Latrive, A.
Castro, P. A. A.
Rossi, W.
Zorn, T. M. T.
Samad, R. E.
Freitas, A. Z.
Cesar, C. L.
Vieira Junior, N. D.
Zezell, D. M.
Abstract: Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma.
Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an in vivo animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy. 3 dorsal areas of 20 anesthetized Wistar rats were burned by water vapor exposure and subsequently treated either by classical surgical debridement, by laser ablation, or left without treatment. Skin burn tissues were non-invasively characterized by OCT images and biopsied for further histopathology analysis, SHG imaging and FTIR spectroscopy at 3, 5, 7 and 14 days after burn. The laser protocol was found as efficient as the classical treatment for promoting the healing process. The study concludes to the validation of femtosecond ultra-short pulses laser treatment for skinburns, with the advantage of minimizing operatory trauma.
Subject: Tomografia de coerência óptica
Transformadas de Fourier
Microscopia
Country: Estados Unidos
Editor: Optical Society of America
Citation: Biomedical Optics Express. Optical Soc Amer, v. 8, p. 1575 - 1588, 2017.
Rights: aberto
Identifier DOI: 10.1364/BOE.8.001575
Address: https://www.osapublishing.org/boe/abstract.cfm?uri=boe-8-3-1575
Date Issue: 2017
Appears in Collections:IFGW - Artigos e Outros Documentos

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