Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328801
Type: Artigo
Title: Influence Of The Culture Medium In Dose-response Effect Of The Chlorhexidine On Streptococcus Mutans Biofilms
Author: de Queiroz
Vanessa Salvadego; Ccahuana-Vasquez
Renzo Alberto; Tedesco
Alcides Fabiano; Lyra
Luzia; Cury
Jaime Aparecido; Schreiber
Angelica Zaninelli
Abstract: The aim of this study was to evaluate the influence of culture medium on dose-response effect of chlorhexidine (CHX) on Streptococcus mutans UA159 biofilm and validate the use of the cation-adjusted-Muller-Hinton broth (MH) for the evaluation of antibacterial activity. Ultrafiltered Tryptone-Yeast Extract Broth (UTYEB) was compared against MH and MH with blood supplementation (MHS). For each medium, six groups (n = 4) were assessed: two negative control groups (baseline 48 and 120 h) and four experimental groups (0.0001, 0.001, 0.012, and 0.12% CHX). S. mutans biofilm grewon glass slides of each media containing 1% sucrose. After 48 h of growth, biofilms of baseline 48 h were collected and the other groups were treated for 1 min, twice a day, for 3 days, with their respective treatments. The media were changed daily and pH was measured. After 120 h, biofilms were collected and dry weight and viable microorganisms were determined. Results showed CHX dose-response effect being observed in all media for all the variables. However, MH and MHS showed higher sensitivity than UTYEB (p < 0.05). We can conclude that the culture medium does influence dose-response effect of CHX on Streptococcus mutans biofilm and that MH can be used for antibacterial activity.
Subject: Dental-caries
Gene-expression
Oral Biofilm
Plaque
Sucrose
Demineralization
Cariogenicity
Inhibition
Editor: Hindawi Publishing Corp
New York
Rights: aberto
Identifier DOI: 10.1155/2016/2816812
Address: https://www.hindawi.com/journals/scientifica/2016/2816812/
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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