Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328764
Type: Artigo
Title: Variation In Olfactory Neuron Repertoires Is Genetically Controlled And Environmentally Modulated
Author: Ibarra-Soria
Ximena; Nakahara
Thiago S.; Lilue
Jingtao; Jiang
Yue; Trimmer
Casey; Souza
Mateus A. A.; Netto
Paulo H. M.; Ikegami
Kentaro; Murphy
Nicolle R.; Kusma
Mairi; Kirton
Andrea; Saraiva
Luis R.; Keane
Thomas M.; Matsunami
Hiroaki; Mainland
Joel; Papes
Fabio; Logan
Darren W.
Abstract: The mouse olfactory sensory neuron (OSN) repertoire is composed of 10 million cells and each expresses one olfactory receptor (OR) gene from a pool of over 1000. Thus, the nose is sub-stratified into more than a thousand OSN subtypes. Here, we employ and validate an RNA-sequencing-based method to quantify the abundance of all OSN subtypes in parallel, and investigate the genetic and environmental factors that contribute to neuronal diversity. We find that the OSN subtype distribution is stereotyped in genetically identical mice, but varies extensively between different strains. Further, we identify cis-acting genetic variation as the greatest component influencing OSN composition and demonstrate independence from OR function. However, we show that olfactory stimulation with particular odorants results in modulation of dozens of OSN subtypes in a subtle but reproducible, specific and time-dependent manner. Together, these mechanisms generate a highly individualized olfactory sensory system by promoting neuronal diversity.
Subject: Mammalian Odorant Receptors
Copy-number Variation
Gene-expression
Functional Expression
Sensory Neurons
Molecular-basis
In-vivo
Mouse
Mice
Perception
Editor: Elife Sciences Publications Ltd
Cambridge
Rights: aberto
Identifier DOI: 10.7554/eLife.21476
Address: https://elifesciences.org/articles/21476
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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