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Type: Artigo
Title: Zika Virus Disrupts Molecular Fingerprinting Of Human Neurospheres
Author: Garcez
Patricia P.; Nascimento
Juliana Minardi; de Vasconcelos
Janaina Mota; da Costa
Rodrigo Madeiro; Delvecchio
Rodrigo; Trindade
Pablo; Loiola
Erick Correia; Higa
Luiza M.; Cassoli
Juliana S.; Vitoria
Gabriela; Sequeira
Patricia C.; Sochacki
Jaroslaw; Aguiar
Renato S.; Fuzii
Hellen Thais; Bispo de Filippis
Ana M.; Goncalves Vianez Junior
Joao Lidio da Silva; Tanuri
Amilcar; Martins-de-Souza
Daniel; Rehen
Stevens K.
Abstract: Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human brain development are still not fully understood. Here we describe alterations in human neurospheres derived from induced pluripotent stem (iPS) cells infected with the strain of Zika virus that is circulating in Brazil. Combining proteomics and mRNA transcriptional profiling, over 500 proteins and genes associated with the Brazilian ZIKV infection were found to be differentially expressed. These genes and proteins provide an interactome map, which indicates that ZIKV controls the expression of RNA processing bodies, miRNA biogenesis and splicing factors required for self-replication. It also suggests that impairments in the molecular pathways underpinning cell cycle and neuronal differentiation are caused by ZIKV. These results point to biological mechanisms implicated in brain malformations, which are important to further the understanding of ZIKV infection and can be exploited as therapeutic potential targets to mitigate it.
Subject: Pluripotent Stem-cells
Large Gene Lists
Phylogenetic Analysis
Neuronal Development
Editor: Nature Publishing Group
Rights: aberto
Identifier DOI: 10.1038/srep40780
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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