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Type: Artigo
Title: De Novo Aml Exhibits Greater Microenvironment Dysregulation Compared To Aml With Myelodysplasia-related Changes
Author: Lopes
Matheus Rodrigues; Novais Pereira
Joao Kleber; Campos
Paula de Melo; Machado-Neto
Joao Agostinho; Traina
Fabiola; Olalla Saad
Sara T.; Favaro
Abstract: The interaction between the bone marrow microenvironment and malignant hematopoietic cells can result in the protection of leukemia cells from chemotherapy in both myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). We, herein, characterized the changes in cytokine expression and the function of mesenchymal stromal cells (MSC) in patients with MDS, AML with myelodysplasia-related changes (MRC), a well-recognized clinical subtype of secondary AML, and de novo AML. We observed a significant inhibitory effect of MDS-MSC on T-lymphocyte proliferation and no significant differences in any of the cytokines tested. AML-MSC inhibited T-cell proliferation only at a very low MSC/T cell ratio. When compared to the control, AML-MRC-derived MSC presented a significant increase in IL6 expression, whereas de novo AML MSC presented a significant increase in the expression levels of VEGFA, CXCL12, RPGE2, IDO, IL1 beta, IL6 and IL32, followed by a decrease in IL10 expression. Furthermore, data indicate that IL-32 regulates stromal cell proliferation, has a chemotactic potential and participates in stromal cell crosstalk with leukemia cells, which could result in chemoresistance. Our results suggest that the differences between AML-MRC and de novo AML also extend into the leukemic stem cell niche and that IL-32 can participate in the regulation of the bone marrow cytokine milieu.
Subject: Acute Myeloid-leukemia
Mesenchymal Stromal Cells
Hematopoietic Progenitors
Proinflammatory Cytokine
Editor: Nature Publishing Group
Citation: Scientific Reports. Nature Publishing Group, v. 7, p. , 2017.
Rights: aberto
Identifier DOI: 10.1038/srep40707
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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